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Series GSE89313 Query DataSets for GSE89313
Status Public on Nov 21, 2016
Title Tet-dependent transcriptional programs during early embryogenesis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To determine the transcriptional effects of lack of Tet proteins during early embryogenesis, we performed single-embryo RNA-sequencing of control and TKO embryos (E6.75; 4 embryos from each group). Genome-wide analyses showed that Tet deficiency promotes the expression of mesoderm-related genes during early embryogenesis in vivo.
Overall design Control and Tet1/2/3 TKO embryos were collected at E6.75. Single embryos were lysed and converted into double stranded cDNA using SMARTer ultra-low RNA kit for Illumina sequencing (Clontech). 1 ng of cDNA for each sample was used for preparing libraries using Nextera XT DNA sample preparation kit (IIlumina). Libraries were sequenced on Illumina HiSeq 2500 platforms according to the manufacturer's instructions.
Contributor(s) Li X, Rao A
Citation(s) 27930333
Submission date Oct 30, 2016
Last update date May 15, 2019
Contact name ANJANA RAO
Organization name La Jolla Institute for Allergy and Immunology
Street address 9420 Athena Circle
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (8)
GSM2365886 CTL embryo, replicate 1
GSM2365887 CTL embryo, replicate 2
GSM2365888 CTL embryo, replicate 3
This SubSeries is part of SuperSeries:
GSE89316 Tet proteins influence the balance between neuroectodermal and mesodermal fate choice by inhibiting Wnt signaling
BioProject PRJNA351757
SRA SRP092291

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89313_EmbryoCell_RPKM.txt.gz 424.9 Kb (ftp)(http) TXT
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