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Series GSE89232 Query DataSets for GSE89232
Status Public on Nov 15, 2016
Title Single-cell RNA-seq reveals heterogeneity within human pre-cDCs
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Here, we use a microfluidics-based approach to prepare single-cell RNA-Seq libraries from 164 primary human conventional dendritic cells (cDCs) as well as cord blood (452) and blood (341) pre-cDCs. We examined heterogeneity between individual cells to document potential subpopulations within human cDCs and pre-cDCs.
 
Overall design RNA seq libraries from 957 single cells were prepared using the SMART-Seq 2 protocol (Picelli et al., 2013)
341 Blood pre-cDC (Blood pre-cDC_S* data columns in the expMatrix.txt)
452 CB pre-cDC (CB pre-cDC_S* data columns in the expMatrix.txt)
87 CD141+ cDC (blood_bdca3_S* data columns in the expMatrix.txt)
77 CD1c+ cDC (blood_bdca1_S* data columns in the expMatrix.txt)
 
Contributor(s) Oliveira T, Satija R
Citation(s) 27864467
Submission date Oct 27, 2016
Last update date Mar 20, 2019
Contact name Thiago Y Oliveira
E-mail toliveira@rockefeller.edu
Organization name The Rockefeller University
Department Immunology, Virology and Microbiology
Lab Laboratory of Molecular Immunology
Street address 1230 YORK AVE
City NEW YORK
State/province New York
ZIP/Postal code 10065
Country USA
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (957)
GSM2361495 CD1c+ cDC_S1
GSM2361496 CD1c+ cDC_S2
GSM2361497 CD1c+ cDC_S3
Relations
BioProject PRJNA350790
SRA SRP092166

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89232_expMatrix.txt.gz 29.5 Mb (ftp)(http) TXT
Raw data are available in SRA
Processed data is available on Series record

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