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Status |
Public on Nov 15, 2016 |
Title |
Single-cell RNA-seq reveals heterogeneity within human pre-cDCs |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Here, we use a microfluidics-based approach to prepare single-cell RNA-Seq libraries from 164 primary human conventional dendritic cells (cDCs) as well as cord blood (452) and blood (341) pre-cDCs. We examined heterogeneity between individual cells to document potential subpopulations within human cDCs and pre-cDCs.
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Overall design |
RNA seq libraries from 957 single cells were prepared using the SMART-Seq 2 protocol (Picelli et al., 2013) 341 Blood pre-cDC (Blood pre-cDC_S* data columns in the expMatrix.txt) 452 CB pre-cDC (CB pre-cDC_S* data columns in the expMatrix.txt) 87 CD141+ cDC (blood_bdca3_S* data columns in the expMatrix.txt) 77 CD1c+ cDC (blood_bdca1_S* data columns in the expMatrix.txt)
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Contributor(s) |
Oliveira T, Satija R |
Citation(s) |
27864467 |
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Submission date |
Oct 27, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Thiago Y Oliveira |
E-mail(s) |
toliveira@rockefeller.edu
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Organization name |
The Rockefeller University
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Department |
Immunology, Virology and Microbiology
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Lab |
Laboratory of Molecular Immunology
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Street address |
1230 YORK AVE
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City |
NEW YORK |
State/province |
New York |
ZIP/Postal code |
10065 |
Country |
USA |
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Platforms (1) |
GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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Samples (957)
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Relations |
BioProject |
PRJNA350790 |
SRA |
SRP092166 |