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| Status |
Public on Nov 15, 2016 |
| Title |
Single-cell RNA-seq reveals heterogeneity within human pre-cDCs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Here, we use a microfluidics-based approach to prepare single-cell RNA-Seq libraries from 164 primary human conventional dendritic cells (cDCs) as well as cord blood (452) and blood (341) pre-cDCs. We examined heterogeneity between individual cells to document potential subpopulations within human cDCs and pre-cDCs.
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| Overall design |
RNA seq libraries from 957 single cells were prepared using the SMART-Seq 2 protocol (Picelli et al., 2013)
341 Blood pre-cDC (Blood pre-cDC_S* data columns in the expMatrix.txt)
452 CB pre-cDC (CB pre-cDC_S* data columns in the expMatrix.txt)
87 CD141+ cDC (blood_bdca3_S* data columns in the expMatrix.txt)
77 CD1c+ cDC (blood_bdca1_S* data columns in the expMatrix.txt)
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| |
|
| Contributor(s) |
Oliveira T, Satija R |
| Citation(s) |
27864467 |
| Submission date |
Oct 27, 2016 |
| Last update date |
Mar 20, 2019 |
| Contact name |
Thiago Y Oliveira |
| E-mail |
toliveira@rockefeller.edu
|
| Organization name |
The Rockefeller University
|
| Department |
Immunology, Virology and Microbiology
|
| Lab |
Laboratory of Molecular Immunology
|
| Street address |
1230 YORK AVE
|
| City |
NEW YORK |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (957)
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|
| Relations |
| BioProject |
PRJNA350790 |
| SRA |
SRP092166 |
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