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Series GSE89144 Query DataSets for GSE89144
Status Public on Jan 24, 2017
Title Mercury-Induced Epigenetic Transgenerational Inheritance of Abnormal Neurobehavior is Correlated with Sperm Epimutations in Zebrafish
Organism Danio rerio
Experiment type Methylation profiling by high throughput sequencing
Summary Methylmercury (MeHg) is a ubiquitous environmental neurotoxicant, with human exposures predominantly resulting from fish consumption. Developmental exposure of zebrafish to MeHg is known to alter their neurobehavior. The current study investigated the direct exposure and transgenerational effects of MeHg, at tissue doses similar to those detected in exposed human populations, on sperm epimutations (i.e., differential DNA methylation regions [DMRs]) and neurobehavior (i.e., visual startle and spontaneous locomotion) in zebrafish, an established human health model. F1 generation embryos were exposed to MeHg (0, 1, 3, 10, 30, and 100 nM) for 24 hours ex vivo. F1 generation control and MeHg-exposed lineages were reared to adults and bred to yield the F2 generation, which was subsequently bred to the F3 generation. Direct exposure (F1 generation) and transgenerational actions (F3 generation) were then evaluated. Hyperactivity and visual deficit were observed in the unexposed descendants (F3 generation) of the MeHg-exposed lineage compared to control. An increase in F3 generation sperm epimutations was observed relative to the F1 generation. Investigation of the DMRs in the F3 generation MeHg-exposed lineage sperm revealed associated genes in the neuroactive ligand-receptor interaction and actin-cytoskeleton pathways being effected, which correlate to the observed neurobehavioral phenotypes. Developmental MeHg-induced epigenetic transgenerational inheritance of abnormal neurobehavior is correlated with sperm epimutations in F3 generation adult zebrafish. Therefore, mercury has the ability to promote the epigenetic transgenerational inheritance of disease in zebrafish, which significantly impacts its environmental health considerations in all species including humans.
Overall design F1 generation zebrafish embryos were exposed to MeHg. F1 generation control and MeHg-exposed lineages propogated to the F3 generation. Sperm was collected from both F1 and F3 generation fish. The sperm from two fish was combined to create a single pooled sample. Three pools were collected for both control and MeHg treated groups. The methylation characteristics of the pooled samples was estimated using a methylated DNA immunoprecipitation (MeDIP) followed by PE50 sequencing on an Illumina HiSeq 2500 platform.
Contributor(s) Carvan III MJ, Kalluvila TA, Klingler RH, Larson JK, Pickens M, Mora-Zamorano FX, Connaughton VP, Sadler-Riggleman I, Beck D, Skinner MK
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Submission date Oct 25, 2016
Last update date May 15, 2019
Contact name Michael K Skinner
Organization name WSU
Department SBS
Street address Abelson 507
City Pullman
State/province WA
ZIP/Postal code 99163
Country USA
Platforms (1)
GPL18413 Illumina HiSeq 2500 (Danio rerio)
Samples (12)
GSM2359639 F1P1
GSM2359640 F1P2
GSM2359641 F1P3
BioProject PRJNA350395
SRA SRP092038

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Supplementary file Size Download File type/resource
GSE89144_F1.results.csv.gz 649.0 Mb (ftp)(http) CSV
GSE89144_F3.results.csv.gz 685.2 Mb (ftp)(http) CSV
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Processed data are available on Series record
Raw data are available in SRA

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