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Series GSE89106 Query DataSets for GSE89106
Status Public on Aug 04, 2017
Title Targeting Mycobacterium tuberculosis Sensitivity to Thiol Stress at Acidic pH Kills the Bacterium and Potentiates Antibiotics
Organism Mycobacterium tuberculosis CDC1551
Experiment type Expression profiling by high throughput sequencing
Summary The purpose of this study was to characterize the mechanism of action of the small molecule AC2P36
Overall design Mtb strain CDC1551 was grown at 37ºC in T-25 vented, standing tissue culture flasks in 8 mL of buffered 7H9 medium (pH 7.0 or 5.7) lacking catalase to an OD600 of 0.5. Cultures were subsequently treated with 10 µM AC2P36. For the control, cultures treated with an equal amount of DMSO. Following a 4 h incubation period, total bacterial RNA was extracted, quality controlled and sequenced. Each treatment was repeated with two independent (biological) replicates.
Contributor(s) Coulson GB, Johnson BK, Colvin CJ, Fillinger RJ, Zheng H, Haiderer E, Hammer ND, Abramovitch RB
Citation(s) 28781126
Submission date Oct 24, 2016
Last update date Nov 03, 2017
Contact name Robert B. Abramovitch
Organization name Michigan State University
Department Microbiology and Molecular Genetics
Street address 567 Wilson Road
City East Lansing
State/province MI
ZIP/Postal code 48824
Country USA
Platforms (1)
GPL20114 Illumina HiSeq 2500 (Mycobacterium tuberculosis CDC1551)
Samples (8)
GSM2358864 WT treated with DMSO, pH 7.0 rep 1
GSM2358865 WT treated with DMSO, pH 7.0 rep 2
GSM2358866 WT treated with DMSO, pH 5.7 rep 1
BioProject PRJNA350293
SRA SRP091985

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89106_Table_S1.xlsx 793.7 Kb (ftp)(http) XLSX
GSE89106_Table_S2.xlsx 803.8 Kb (ftp)(http) XLSX
GSE89106_Table_S3.xlsx 828.6 Kb (ftp)(http) XLSX
Raw data are available in SRA
Processed data is available on Series record

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