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Series GSE8836 Query DataSets for GSE8836
Status Public on Dec 01, 2007
Title CLL in Em-TCL1 mice provides a biologically relevant model to unravel and reverse immune deficiency in human cancer.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Immune deficiency is common in cancer, but the biological basis for this and ways to reverse it remains elusive. Here we present a mouse model of B cell chronic lymphocytic leukemia (CLL) that recapitulates changes in the non-malignant circulating T cells seen in patients with this illness.1 To validate this model, we examined changes in T cell gene expression, protein expression and function in Em-TCL1 transgenic mice as they developed CLL 2,3 and demonstrate that development of CLL in these transgenic mice is associated with changes in impaired T cell function and in gene expression in CD4 and CD8 T cells similar to those observed in patients with this disease. Infusion of CLL cells into non-leukemia bearing Em-TCL1 mice rapidly induces these changes, demonstrating a causal relationship between leukemia and the induction of T cell changes. This model allows dissection of the molecular changes induced in CD4 and CD8 T cells by interaction with leukemia cells and further supports the concept that cancer results in complex abnormalities in the immune microenvironment.
Gene expression profiling was performed to determine whether Em-TCL1 murine model of chronic lymphocytic leukemia (CLL) mimics T cell defects induced by CLL cells in patients with CLL.
Keywords: comparative gene expression profiling analysis.
 
Overall design CD4 T cells and CD8 T cells were obtained from spleens of B6C3 and Em-TCL1 transgenic murine model of CLL or from peripheral blood mononuclear cells of previously untreated patients with CLL and healthy individuals (Pubmed ID: 15965501). Gene expression profiling was performed using total RNA and the data were analysed to compare gene expression profile of CLL to healthy within or between the species.
 
Contributor(s) Görgün G, Holderried TA, Ramsay AG, Zahrieh D, Liu F, Quackenbush J, Croce CM, Gribben JG
Citation(s) 19332800
Submission date Aug 21, 2007
Last update date Feb 11, 2019
Contact name Gullu Gorgun
E-mail gullu_gorgun@dfci.harvard.edu
Phone 617 632 3358
Fax 617 632 3222
Organization name Dana-Farber Cancer Institute
Department Medical Oncology
Street address 44 Binney Street
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (56)
GSM219522 WT1-CD4
GSM219523 WT2-CD4
GSM219524 WT3-CD4
Relations
BioProject PRJNA102173

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8836_RAW.tar 355.0 Mb (http)(custom) TAR (of CEL, TXT)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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