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Series GSE87618 Query DataSets for GSE87618
Status Public on Jul 13, 2017
Title Identifying ASCL1 target genes in primary GBM stem cell cultures [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary ASCL1 mediates neuronal differentiation of GBM stem cell (GSC) cultures. We sought to identify genomic targets of ASCL1 in primary human GSC cultures. In this dataset, we include ChIP-seq data obtained from GSC cultures harbouring a CRISPR-deletion of ASCL1. We assessed differential ASCL1 binding between control and GSC cultures induced to overexpress ASCL1 after 18 hours of doxycycline treatment.
Overall design Glioblastoma cells with a genetic knockout of ASCL1 is compared to genetic knockout of ASCL1 overexpressing ASCL1 (n = 3 biological replicates; n = 2 input samples; total = 8 samples)
Contributor(s) Park NI, Guilhamon P, Lupien M, Dirks PB
Citation(s) 28712938
Submission date Oct 04, 2016
Last update date May 15, 2019
Contact name Peter B Dirks
Organization name The Hospital for Sick Children
Department Arthur and Sonia Labatt Brain Tumour Research Centre and Division of Neurosurgery
Street address 686 Bay St
City Toronto
State/province ON
ZIP/Postal code M5G 0A4
Country Canada
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (8)
GSM2335528 ASCL1 ChIPseq negative control rep1
GSM2335529 ASCL1 ChIPseq negative control rep2
GSM2335530 ASCL1 ChIPseq negative control rep3
BioProject PRJNA345388
SRA SRP090832

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Supplementary file Size Download File type/resource
GSE87618_RAW.tar 170.0 Kb (http)(custom) TAR (of NARROWPEAK)
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Raw data are available in SRA
Processed data provided as supplementary file

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