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Series GSE87571 Query DataSets for GSE87571
Status Public on Oct 04, 2016
Title Continuous Aging of the Human DNA Methylome Throughout the Human Lifespan
Organism Homo sapiens
Experiment type Methylation profiling by high throughput sequencing
Summary DNA methylation plays an important role in development of disease and the process of aging. In this study we examine DNA methylation at 476,366 sites throughout the genome of white blood cells from a population cohort (N = 421) ranging in age from 14 to 94 years old. Age affects DNA methylation at almost one third (29%) of the sites (Bonferroni adjusted P-value <0.05), of which 60.5% becomes hypomethylated and 39.5% hypermethylated with increasing age. DNA methylation sites that are located within CpG islands (CGIs) more often become hypermethylated compared to sites outside an island. CpG sites in promoters are more unaffected by age, whereas sites in enhancers more often becomes hypo- or hypermethylated. Hypermethylated sites are overrepresented among genes that are involved in DNA binding, transcription regulation, processes of anatomical structure and developmental process and cortex neuron differentiation (P-value down to P = 9.14*10−67). By contrast, hypomethylated sites are not strongly overrepresented among any biological function or process. Our results indicate that the 23% of the variation in DNA methylation is attributed chronological age, and that hypermethylation is more site-specific than hypomethylation. It appears that the change in DNA methylation partly overlap with regions that change histone modifications with age, indicating an interaction between the two major epigenetic mechanisms. Epigenetic modifications and change in gene expression over time most likely reflects the natural process of aging and variation between individuals might contribute to the development of age-related phenotypes and diseases such as type II diabetes, autoimmune and cardiovascular disease.
 
Overall design Bisulphite converted DNA from 732 samples were hybridized to the Illumina Infinium 450K Human Methylation Beadchip
 
Contributor(s) Johansson Å
Citation(s) 23826282
Submission date Oct 03, 2016
Last update date Mar 22, 2019
Contact name Åsa Johansson
E-mail asa.johansson@igp.uu.se
Organization name Uppsala University
Department IGP
Street address BMC, Husarg 3
City Uppsala
State/province Sweden
ZIP/Postal code 75108
Country Sweden
 
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (732)
GSM2333901 X1 genomic DNA from whole blood
GSM2333902 X2 genomic DNA from whole blood
GSM2333903 X3 genomic DNA from whole blood
Relations
BioProject PRJNA345217

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE87571_RAW.tar 6.1 Gb (http)(custom) TAR (of IDAT)
GSE87571_additional_sample_chararcteristics.xlsx 1.0 Mb (ftp)(http) XLSX
GSE87571_matrix1of2.txt.gz 1.4 Gb (ftp)(http) TXT
GSE87571_matrix2of2.txt.gz 1.4 Gb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data is available on Series record

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