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Series GSE8741 Query DataSets for GSE8741
Status Public on Nov 21, 2007
Title DELLA protein direct targets in Arabidopsis
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary The aim of this study is to identify early DELLA protein-responsive genes using a Dexamethasone (DEX)-inducible system. Two transgenic lines were used: one induces the expression of a dominant, gibberellin non-responsive DELLA protein (rga-delta17); the other is a control line that carries the same vector, but lacks the rga-delta17 transgene. By comparing the gene expression changes in the line that expresses the rga-delta17 protein in the presence or absence of DEX it is possible to identify putative targets of DELLA proteins. An empty vector transgenic line was included in this study to identify genes that might be regulated by the DEX inducible system that are not dependent on the DELLA protein.
Keywords: Dexamethasone treatment, gibberellin treatment, time course, transgene effect
 
Overall design Seedlings of both transgenic lines were pretreated for 16 h with 2 uM GA4 to enhance gibberellin responses. Because DELLA proteins are strong signaling repressors, this pretreatment should maximize the effect of DELLA induction. Eight-day old seedlings were treated with 2 uM GA4 or a combination of 2 uM GA4 plus 10 uM DEX to induce the rga-delta17 transgene. Three biological replicas for the transgenic line that carries the DEX-inducible rga-delta17 transgene were generated at 2h and 4h. For the empty vector line, only 2 biological replicas were generated at 4h of treatment with 2 uM GA with or without 10 uM DEX.
 
Contributor(s) Zentella R, Zhang Z, Park M, Thomas SG, Endo A, Murase K, Fleet CM, Jikumaru Y, Nambara E, Kamiya Y, Sun T
Citation(s) 17933900
Submission date Aug 09, 2007
Last update date Aug 28, 2018
Contact name Rodolfo Zentella
E-mail(s) zentella@duke.edu
Phone 9196138222
Fax 9196607293
Organization name Duke University
Department Biology
Street address Box 90338, Science Dr
City Durham
State/province NC
ZIP/Postal code 27708
Country USA
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (16)
GSM216934 Dex-rga(delta17)_shoots_2h_GA4_repl1
GSM216938 Dex-rga(delta17)_shoots_2h_GA4_repl2
GSM216943 Dex-rga(delta17)_shoots_2h_GA4_repl3
This SubSeries is part of SuperSeries:
GSE8785 Early gibberellin responses and DELLA protein direct targets in Arabidopsis
Relations
BioProject PRJNA105285

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8741_RAW.tar 56.6 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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