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| Status |
Public on Jan 01, 2018 |
| Title |
Omeprazole regulate gastric cancer microenvironment by regulate exosomes associated miRNA |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Exosomal miRNAs have emerged as microcommunicators of pathologic conditions including cancer where they educate the tumor microenvironment in favor of metastasis. We purified exosomes from the medium of SCG-7901 cells which was treated by 80ug/ml omeprazole 24h and was analysed by Aglient human microarray.
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| Overall design |
SGC-7901 cells was treated with omeprazole at 80ug/ml for 24h, and PBS was used as control. And the medium of SGC-7901 cells were collected and the exosomes were isolated by ExoQuick-TC exosome precipitation solution. Then the miRNA involved in exosomes was isolated by the total RNA purification Kit.Next the miRNA was analysised by microarry.
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| Contributor(s) |
Guan X, Huang D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Sep 21, 2016 |
| Last update date |
Jan 02, 2018 |
| Contact name |
Xu-wen Guan |
| E-mail(s) |
guanxuwen@tmu.edu.cn
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| Phone |
+8617702250916
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| Organization name |
Tianjin Medical University
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| Street address |
Huanhuxi Road, Tiyuanbei, Hexi District
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| City |
Tianjin |
| State/province |
Tianjin |
| ZIP/Postal code |
300060 |
| Country |
China |
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| Platforms (1) |
| GPL19730 |
Agilent-046064 Unrestricted_Human_miRNA_V19.0_Microarray (Probe Name version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA343725 |
