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| Status |
Public on Jan 21, 2019 |
| Title |
Myc activation in breast cancer is due to p53-loss and sustains self-renewal of cancer stem cells (ChIP-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Purpose: discover the downstream pathways and the mechanism of target activation by the p53:Myc axis in normal mammary and cancer stem cells
Methods: we performed ChIPseq experiments from NMuMG cells grown in adhesion under standard conditions
Results: the p53:Myc axis orchestrates its transcriptional response in mammary-epithelial cells via a dual and overlapping mechanism: i) a tightly controlled epistasis driven by p53 and executed by Myc, and ii) a co-operative double occupancy of their downstream effectors at different regulatory regions
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| |
|
| Overall design |
Examination p53 and Myc binding sites in murine mammary epithelial cells
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| |
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| Contributor(s) |
Santoro A, Vlachou T, Luzi L, Pasi C, Reavie L, Melloni G, Bonetti P, Pelicci PG |
| Citation(s) |
30650356 |
| |
| Submission date |
Sep 16, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Pier Giuseppe Pelicci |
| Organization name |
European Institute of Oncology
|
| Department |
Department of Experimental Oncology
|
| Street address |
Via Adamello, 16
|
| City |
Milano |
| ZIP/Postal code |
20139 |
| Country |
Italy |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE87004 |
Myc activation in breast cancer is due to p53-loss and sustains self-renewal of cancer stem cells |
|
| Relations |
| BioProject |
PRJNA343203 |
| SRA |
SRP089996 |
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