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Series GSE86924 Query DataSets for GSE86924
Status Public on Sep 15, 2016
Title Effect of NR4A3 siRNA treatment on gene expression of islet derived hybrid cell of 1.1B4
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Gene regulation associated with antioxidant defense system in pancreatic beta cells still needs to be elucidated, we found NR4A3 gene expression was up-regulated with 100mM H2O2, therefore, we analyzed the genes expression with Agilent micro-array after NR4A3 siRNA4 treatment by one color method, and found 1044 genes were up-regulated and 859 genes were down regulated in NR4A3 knocked down cell. Changes of some genes expressions were confirmed by real time PCR.
The genes of 1044 in NR4A3 siRNA treated cell were up-regulated over 2 folds and 859 genes were down-regulated under 0.67 folds compared with control sample.
 
Overall design We analyzed the comprehensive gene expression changes treated by NR4A3 siRNA in pancreatic beta-cell derived 1.1B4 cells using Agilent microarray.
 
Contributor(s) Otsuka Y, Yamada M, Ueta E
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Submission date Sep 14, 2016
Last update date Jan 09, 2018
Contact name Yuzuru Otsuka
E-mail(s) otsuka@toita.ac.jp
Organization name Toita Women's College
Department Department of Nutrition
Street address 2-21-17 Shiba
City Minato-ku
State/province Tokyo
ZIP/Postal code 105-0014
Country Japan
 
Platforms (1)
GPL13497 Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version)
Samples (8)
GSM2310360 Human_islet_1_1B4_cell_control1
GSM2310361 Human_islet_1_1B4_NR4A3siRNA_48hr1
GSM2310362 Human_islet_1_1B4_cell_control2
Relations
BioProject PRJNA343006

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE86924_RAW.tar 71.3 Mb (http)(custom) TAR (of TXT)
GSE86924_normalized_data_full.txt.gz 1.7 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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