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| Status |
Public on Sep 17, 2016 |
| Title |
A total RNA-Seq screen reveals that the splicing inhibitor Isoginkgetin blocks transcription elongation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Pharmacological perturbation is a powerful tool for understanding gene expression, but identification of the specific steps of this multi-step process targeted by small molecules remains challenging. Here we apply total RNA-Seq to distinguish specific pharmacological effects on transcription and pre-mRNA processing, revealing unexpectedly that the splicing inhibitor isoginkgetin blocks transcription elongation.An RNA-Seq screen reveals that the compounds TBBz and CYT387 mimic the transcriptional effects of isoginkgetin and are inhibitors of CSNK2A2, suggesting that isoginkgetin blocks transcription elongation via inhibition of CSNK2A2. Our results reveal RNA-Seq screening as a tool for disentangling complex pharmacological effects on gene expression.
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| |
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| Overall design |
Total RNA sequencing of drug treated Hela cells for 6 or 18 hours.
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| |
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| Contributor(s) |
Gray JM, Springer M, Boswell SA |
| Citation(s) |
28263964 |
| |
| Submission date |
Sep 13, 2016 |
| Last update date |
Jun 15, 2020 |
| Contact name |
jesse M gray |
| E-mail(s) |
jesse.gray@gmail.com
|
| Phone |
6174321877
|
| Organization name |
Harvard Medical School
|
| Department |
Genetics
|
| Lab |
Gray lab
|
| Street address |
77 avenue louis pasteur, NRB 356
|
| City |
boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (80)
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| Relations |
| BioProject |
PRJNA343235 |
| SRA |
SRP091854 |
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