|
| Status |
Public on Mar 01, 2017 |
| Title |
ChIP-seq of ER and RUNX2 in MCF7 breast cancer cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
We performed ChIP-seq on MCF7 cells in an effort to discern differential binding patterns between wild type MCF7s and MCF7s that have acquired resistance to Tamoxifen and Estrogen deprivation. Furthermore, we also performed an HAtag ChIP on a DOX inducible RUNX2 overexpression MCF7 cell line.
We also performed RNA-seq on MCF7 cells to determine transciptional changes after acquisition of Tamoxifen resistance, and also transciptional changes in a DOX inducible RUNX2 overexpression model.
|
| |
|
| Overall design |
ER and HAtag ChIP on MCF7 cells, RNAseq on MCF7 cells
|
| |
|
| Contributor(s) |
Jeselsohn R, Cornwell M, Pun M |
| Citation(s) |
28507152 |
| |
| Submission date |
Sep 07, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
MacIntosh Grant Cornwell |
| E-mail(s) |
mcornwell1957@gmail.com
|
| Organization name |
New York University
|
| Department |
School of Medicine
|
| Lab |
Ruggles Lab
|
| Street address |
227 E 30th St.
|
| City |
New York City |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (18)
|
|
| Relations |
| BioProject |
PRJNA342148 |
| SRA |
SRP087481 |
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