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Series GSE86538 Query DataSets for GSE86538
Status Public on Mar 01, 2017
Title ChIP-seq of ER and RUNX2 in MCF7 breast cancer cell lines
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary We performed ChIP-seq on MCF7 cells in an effort to discern differential binding patterns between wild type MCF7s and MCF7s that have acquired resistance to Tamoxifen and Estrogen deprivation. Furthermore, we also performed an HAtag ChIP on a DOX inducible RUNX2 overexpression MCF7 cell line.
We also performed RNA-seq on MCF7 cells to determine transciptional changes after acquisition of Tamoxifen resistance, and also transciptional changes in a DOX inducible RUNX2 overexpression model.
 
Overall design ER and HAtag ChIP on MCF7 cells, RNAseq on MCF7 cells
 
Contributor(s) Jeselsohn R, Cornwell M, Pun M
Citation(s) 28507152
Submission date Sep 07, 2016
Last update date May 15, 2019
Contact name MacIntosh Grant Cornwell
E-mail(s) mcornwell1957@gmail.com
Organization name Dana Farber Cancer Institute
Department Medical Oncology
Lab Brown Lab
Street address 450 Brookline Ave
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (18)
GSM2305313 MCF7_E2_ER_ChIPseq
GSM2305314 MCF7_TAMR_noE2_ER_ChIPseq
GSM2305315 MCF7_TAMR_E2_ChIPseq
Relations
BioProject PRJNA342148
SRA SRP087481

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE86538_MCF7_Parental_v_TAMresistant_raw_countmat.csv.gz 214.3 Kb (ftp)(http) CSV
GSE86538_MCF7_RUNX2_NoDOX_v_DOX_raw_countmat.csv.gz 279.3 Kb (ftp)(http) CSV
GSE86538_RAW.tar 3.8 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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