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| Status |
Public on Oct 20, 2016 |
| Title |
HNRNPA2B1 regulates alternative RNA processing in the nervous system and accumulates in granules in ALS IPSC-derived motor neurons [hnRNPA2B1_Arrays_human_iPSC_MN_Stress] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
HnRNPA2B1 encodes an RNA binding protein associated with neurodegenerative disorders. However, its function in the nervous system is unclear. Transcriptome-wide cross-linking and immunoprecipitation in mouse spinal cord discover UAGG motifs enriched within ~2,500 hnRNP A2/B1 binding sites and an unexpected role for hnRNP A2/B1 in alternative polyadenylation. Loss of hnRNP A2/B1 results in alternative splicing, including skipping of an exon in amyotrophic lateral sclerosis (ALS)-associated D-amino acid oxidase (DAO) that reduces D-serine metabolism. Inclusion of the DAO exon is also reduced in transgenic ALS mice models. ALS-associated hnRNP A2/B1 D290V mutant patient fibroblasts and motor neurons differentiated from induced pluripotent stem cells demonstrate gain-of-mutant-dependent splicing differences. Mutant motor neurons also exhibit increased hnRNP A2/B1 localization to cytoplasmic granules during stress, which are abrogated by a small molecule CA43. Our findings and cellular resource identify RNA networks affected in loss of normal and mutated hnRNP A2/B1 with broad relevance to neurodegeneration.
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| Overall design |
Microarray in human iPSC-MNs from patients with hnRNP A2/B1 D290V, VCP R155H, mutations or controls. Samples treated with puromycin or saline. 3 replicates per condition.
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| Contributor(s) |
Martinez FJ, Pratt GA, Van Nostrand E, Batra R, Huelga SC, Kapeli K, Freese P, Chun SJ, Ling K, Gelboin-Burkhart C, Fijany L, Wang H, Nussbacher JK, Kim H, Lardelli R, Sundararaman B, Donohue JP, Lykke-Andersen J, Bennett F, Ares M Jr, Burge CB, Taylor JP, Rigo F, Yeo GW |
| Citation(s) |
27773581 |
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| Submission date |
Sep 06, 2016 |
| Last update date |
Oct 26, 2016 |
| Contact name |
Gene Yeo |
| E-mail(s) |
geneyeo@ucsd.edu
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| Organization name |
UCSD
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| Street address |
2880 Torrey Pines Scenic Dr. Room 3805/Yeo Lab
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL17585 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [probe set (exon) version] |
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| Samples (42)
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| This SubSeries is part of SuperSeries: |
| GSE86464 |
HNRNPA2B1 regulates alternative RNA processing in the nervous system and accumulates in granules in ALS IPSC-derived motor neurons |
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| Relations |
| BioProject |
PRJNA341996 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE86462_RAW.tar |
1.0 Gb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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