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Status |
Public on Feb 01, 2018 |
Title |
Genes and mechanisms regulated by androgens as possible contributors to the male excess observed in autism [LNCaP] |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
We optimized ChIP-sequencing protocole and anti-androgen receptor (AR) antibodies on human prostate cancer cell line (LNCaP) after either DMSO (solvent) or DHT 100nM 4 hours treatment.
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Overall design |
Examination of AR IP sequences with 2 anti-AR antibodies (commercial N-20 from SantaCruz or homemade 3279.4) after DMSO or DHT 100nM treatment by deep sequencing using Illumina HiSeq 2500
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Contributor(s) |
Quartier A, Jost B, Le Gras S, Piton A |
Citation(s) |
29428674 |
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Submission date |
Sep 06, 2016 |
Last update date |
Dec 18, 2020 |
Contact name |
Angélique Quartier |
E-mail(s) |
a.quartier@inoviem.com
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Organization name |
IGBMC
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Street address |
1, rue Laurent Fries
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City |
Illkirch |
ZIP/Postal code |
67400 |
Country |
France |
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Platforms (1) |
GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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Samples (6)
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This SubSeries is part of SuperSeries: |
GSE86458 |
Genes and mechanisms regulated by androgens as possible contributors to the male excess observed in autism |
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Relations |
BioProject |
PRJNA341958 |
SRA |
SRP086623 |