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Series GSE86420 Query DataSets for GSE86420
Status Public on Sep 08, 2017
Title Logical modelling of lymphoid and myeloid cell specification and trans-differentiation [ChIPseq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary To identify nez putative transcriptionnal regulations controlling the specification of B cell and Macrophage, we performed ChIPSeq in a pre-B cell line containing an inducible form of the transcription factor C/EBPa (C10). We performed ChIP-seq against Foxo1 in uninduced b cell, and for Ebf1 in uninduced or after several times of C/EBPa induction.
 
Overall design Genome binding/occupancy of transcription factor Pu1 and histones (H3K4me1/2/3 and H3K27me3) by ChIPseq in uninduced Bcell (Control), after induction Cebpa for 6h (Pulse) and after 6 days of wash (Chase).
 
Contributor(s) Collombet S, van Oevelen C, Graf T, Thieffry D
Citation(s) 28584084
Submission date Sep 02, 2016
Last update date May 15, 2019
Contact name samuel Collombet
E-mail samuel.collombet@ens.fr
Organization name Ecole Normale Superieure
Department Biology
Lab Thieffry
Street address 46 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (5)
GSM2302010 C10_Foxo1
GSM2302011 C10_Ebf1
GSM2302012 C10_C/EBPa.03h_Ebf1
Relations
BioProject PRJNA341853
SRA SRP086242

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE86420_RAW.tar 557.8 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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