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Series GSE86420 Query DataSets for GSE86420
Status Public on Sep 08, 2017
Title Logical modelling of lymphoid and myeloid cell specification and trans-differentiation [ChIPseq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary To identify nez putative transcriptionnal regulations controlling the specification of B cell and Macrophage, we performed ChIPSeq in a pre-B cell line containing an inducible form of the transcription factor C/EBPa (C10). We performed ChIP-seq against Foxo1 in uninduced b cell, and for Ebf1 in uninduced or after several times of C/EBPa induction.
Overall design Genome binding/occupancy of transcription factor Pu1 and histones (H3K4me1/2/3 and H3K27me3) by ChIPseq in uninduced Bcell (Control), after induction Cebpa for 6h (Pulse) and after 6 days of wash (Chase).
Contributor(s) Collombet S, van Oevelen C, Graf T, Thieffry D
Citation(s) 28584084
Submission date Sep 02, 2016
Last update date May 15, 2019
Contact name samuel Collombet
Organization name Ecole Normale Superieure
Department Biology
Lab Thieffry
Street address 46 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (5)
GSM2302010 C10_Foxo1
GSM2302011 C10_Ebf1
GSM2302012 C10_C/EBPa.03h_Ebf1
BioProject PRJNA341853
SRA SRP086242

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Supplementary file Size Download File type/resource
GSE86420_RAW.tar 557.8 Mb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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