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| Status |
Public on Mar 08, 2017 |
| Title |
Histone H3 Lysine 4 trimethylation (H3K4me3) ChIP-Seq to identify actively transcribing genes and their active promoter regions in the narrower and wider vanes of primary remiges, respectively |
| Organism |
Gallus gallus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Purpose: One goal of this study is to identify actively transcribing genes in the mesenchyme (pulp) corresponding to the narrow (lateral) and wide (medial) vane of primary remiges. The other is to pinpoint the active promoter regions of these genes.
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| Overall design |
15 primary remige follicles were collected, cut into the lateral and medial halves and shorted treated in 2x CMF to separate epithelium and mesenchyme. The mesenchymal cells were dissociated by 0.35% collagenase digestion. Then we conduct formaldehyde crosslinking, sonication, IP, reverse crosslinking and removal of protein/RNA. The library preparation and sequencing were done at USC epigenome center.
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| |
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| Contributor(s) |
Li A |
| Citation(s) |
28106042 |
| |
| Submission date |
Sep 02, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Ang Li |
| E-mail(s) |
ang.li3@uta.edu
|
| Phone |
6823063485
|
| Organization name |
University of Texas at Arlington
|
| Department |
Kinesiology
|
| Street address |
655 W Mitchell St, SEIR 244
|
| City |
Arlington |
| State/province |
TX |
| ZIP/Postal code |
76010 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19787 |
Illumina NextSeq 500 (Gallus gallus) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE86415 |
Histone H3 Lysine 4 trimethylation (H3K4me3) ChIP-Seq and RNA-seq of narrower and wider vanes of primary remiges |
|
| Relations |
| BioProject |
PRJNA341714 |
| SRA |
SRP084393 |
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