 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Aug 22, 2017 |
| Title |
p53 ChIP-seq in Nutlin-treated HCT116, MCF7, and SJSA cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To identify genomic loci occupied by p53, we performed p53 ChIP-seq analysis of colorectal carcinoma cell line HCT116, breast carcinoma line MCF7, and osteosarcoma line SJSA treated with MDM2 inhibitor Nutlin.
|
| |
|
| Overall design |
HCT116, MCF7, and SJSA cells were treated with either DMSO or Nutlin for 12 hours. Samples from formaldehyde cross-linked cells were subjected to chromatin immunnoprecipitation and recovered DNA was sequenced.
|
| |
|
| Contributor(s) |
Andrysik Z, Pandey A, Espinosa JM |
| Citation(s) |
28904012 |
| |
| Submission date |
Aug 29, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Ahwan Pandey |
| E-mail(s) |
ahwan.pandey@ucdenver.edu
|
| Organization name |
University of Colorado Anschutz Medical Center
|
| Department |
Pharmacology
|
| Lab |
Espinosa Lab
|
| Street address |
12800 E. 19th Ave.
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (9)
|
| GSM2296273 |
MCF7, ChIP-Seq, Input |
| GSM2296274 |
MCF7, ChIP-Seq, DMSO treated |
| GSM2296275 |
MCF7, ChIP-Seq, Nutlin treated |
| GSM2296276 |
SJSA, ChIP-Seq, Input |
| GSM2296277 |
SJSA, ChIP-Seq, DMSO treated |
| GSM2296278 |
SJSA, ChIP-Seq, Nutlin treated |
|
| This SubSeries is part of SuperSeries: |
| GSE86222 |
Identification of a core p53 transcriptional program with highly fractionated tumor suppressive activity |
|
| Relations |
| BioProject |
PRJNA340392 |
| SRA |
SRP083188 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE86164_RAW.tar |
3.3 Gb |
(http)(custom) |
TAR (of BIGWIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...