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| Status |
Public on Mar 31, 2017 |
| Title |
Single-Cell RNA-Seq Analysis Maps Development of Human Germline Cells and Gonadal- Niche Interactions |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Human fetal germ cells (FGCs) are precursors to sperm and eggs and are crucial for maintenance of the species. However, the developmental trajectories and heterogeneity of human FGCs remain largely unknown. Here, we performed single-cell RNA-seq analysis of over 2000 FGCs and their gonadal niche cells in female and male human embryos spanning several developmental stages. We found that the female FGCs undergo four distinct sequential phases characterized by mitosis, retinoic acid signaling, meiotic prophase, and oogenesis. Male FGCs develop through stages of migration, mitosis and cell cycle arrest. Individual embryos of both sexes simultaneously contain several subpopulations, highlighting the asynchronous and heterogeneous nature of FGC development. Moreover, we observed reciprocal signaling interactions between FGCs and their gonadal niche cells, including activation of BMP and Notch signaling pathways. Our work provides key insights into crucial features of human FGCs during their highly ordered mitotic, meiotic, and gametogenetic processes in vivo.
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| Overall design |
Here we performed single cell RNA-seq analysis for 2,167 individual PGCs as well as their gonadal niche cells, covering the developmental stages from 4 to 26 weeks after gestation in both female and male human embryos
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| Contributor(s) |
Li L, Dong J, Yan L, Qiao J, Tang F |
| Citation(s) |
28457750 |
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| Submission date |
Aug 28, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Ji Dong |
| E-mail(s) |
dongji5211314@126.com
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| Organization name |
Peking University
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| Street address |
Yiheyuan 5#
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| City |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (43)
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| Relations |
| BioProject |
PRJNA340388 |
| SRA |
SRP083134 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE86146_RAW.tar |
47.3 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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