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Series GSE8608 Query DataSets for GSE8608
Status Public on Aug 01, 2007
Title MDM from COPD patients and healthy subjects after treatment with LPS or fine and ultrafine particles
Organism Homo sapiens
Experiment type Expression profiling by array
Summary In this study gene expression of monocyte-derived macrophages (MDM) from chronic obstructive pulmonary disease (COPD) patients and healthy subjects was investigated. MDM were treated with LPS, a combination of fine TiO2 and ultrafine Printex90 particles, or remained untreated.
Keywords: disease state analysis
Overall design MDM of 13 COPD patients and 13 healthy subjects were incubated for four hours with LPS (10ng/ml), a combination of fine TiO2 and ultrafine Printex90 (32ug/ml each) or remained untreated. Cells were harvested, counted, and total RNA was isolated (phenol-chloroform extraction) for each subject individually. Total RNA of the 13 individuals from each group (COPD, healthy) and incubation (untreated, LPS, particles) were pooled and hybridized on a seperate array.
Contributor(s) Hofer TP, Frankenberger M, Mages J, Lang R, Meyer P, Hoffmann R, Ziegler-Heitbrock L
Citation(s) 18084737
Submission date Jul 27, 2007
Last update date Jan 08, 2019
Contact name Thomas Hofer
Organization name Helmholtz Zentrum Munich
Department Comprehensive Pneumology Center
Street address Max-Lebsche-Platz 31
City Munich
ZIP/Postal code 81377
Country Germany
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (6)
GSM213464 humanMDM_COPD_untreated
GSM213465 humanMDM_COPD_LPS
GSM213466 humanMDM_COPD_Particles
BioProject PRJNA101779

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8608_RAW.tar 27.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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