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Series GSE8581 Query DataSets for GSE8581
Status Public on May 31, 2008
Title Human Chronic Obstructive Pulmonary Disorder (COPD) Biomarker
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Chronic obstructive pulmonary disease (COPD) is an inflammatory lung disease with complex pathological features and largely unknown etiologies. Identification and validation of biomarkers for this disease could facilitate earlier diagnosis, appreciation of disease subtypes and/or determination of response to therapeutic intervention. To identify gene expression markers for COPD, we performed genome-wide expression profiling of lung tissue from 56 subjects using the Affymetrix U133 Plus 2.0 array. Lung function measurements from these subjects ranged from normal, un-obstructed to severely obstructed. Analysis of differential expression between cases (FEV1<70%, FEV1/FVC<0.7) and controls (FEV1>80%, FEV1/FVC>0.7) identified a set of 65 probe sets representing discrete markers associated with COPD. Correlation of gene expression with quantitative measures of airflow obstruction (FEV1 or FEV1/FVC) identified a set of 220 probe sets. A total of 31 probe sets were identified that showed evidence of significant correlation with quantitative traits and differential expression between cases and controls.
Keywords: Disease state marker
 
Overall design We assessed genome-wide expression patterns in lung tissue specimens derived from 56 subjects. These subjects were undergoing lobectomy for removal of a suspected tumor, and tissue for our studies was derived from histologically normal tissue distant from the tumor margin. Subjects underwent routine spirometry prior to surgery. Low values for both FEV1 and FEV1/FVC are characteristic features of COPD and associated with the severity of disease. For our studies, Cases (n=15) were defined as subjects with FEV1<70% and FEV1/FVC<0.7 and Controls (n=18) as subjects with FEV1>80% and FEV1/FVC>0.7. A majority of the subjects were diagnosed with adenocarcinoma (n=26) or squamous cell carcinoma (n=19), while other tumor types or benign lesions were found in the remaining subjects (n=11).
 
Contributor(s) Bhattacharya S, Srisuma S, Mariani TJ
Citation(s) 18849563
Submission date Jul 25, 2007
Last update date Jan 08, 2019
Contact name Soumyaroop Bhattacharya
E-mail Soumyaroop_Bhattacharya@URMC.rochester.edu
Phone 585-276-4683
Fax 585-276-2642
URL http://lungtranscriptome.bwh.harvard.edu/
Organization name University of Rochester Medical Center
Department Pediatrics
Lab Mariani
Street address 601 Elmwood Avenue Box 850
City Rochester
State/province NY
ZIP/Postal code 14642
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (58)
GSM210004 Human_COPD_Case1
GSM210005 Human_Control1
GSM210006 Human_COPD_Case2
Relations
BioProject PRJNA101729

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8581_RAW.tar 285.3 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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