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Series GSE85769 Query DataSets for GSE85769
Status Public on Aug 18, 2016
Title Expression and exosome incorporation of microRNA by leukemia versus nonmalignant hematopoietic progenitors
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Third-party reanalysis
Summary Exosomes are paracrine regulators of the tumor microenvironment. However, their complex cargo can hamper an understanding of their specific action in target cells. We therefore sought to identify the changes in exosome miRNA content that occur with malignant transformation by comparing two myeloid cancer cell lines to their nonmalignant predecessors, CD34+ human hematopoietic progenitor cells. We employed microarrays to evaluate which miRNA are present at altered concentrations before and after transformation as well as before and after incorporation into exosomes.
Overall design This experiment sought to compare the cells and exosomes of CD34+ nonmalignant progenitors to those of malignant myeloid cell lines HL-60 and MOLM-14. Cells were cultured under 1% O2 conditions for 48 hours, after which cells and exosomes were collected, and miRNA was extracted for microarray comparison. Normalization included comparison to the additional MOLM-14 cell and exosome samples grown at 1% O2 in GSE55025 (GSM1328048, GSM1328049, GSM1328044, GSM1328045). The complete_normalized_data.txt contains 16 data columns (12 new samples + 4 re-analyzed data).
Contributor(s) Kurre P, Huan J, Hornick NI, Harrington CA, Mongoue-Tchokote S, Potter A, Fisher M
Citation(s) 27601730
Submission date Aug 17, 2016
Last update date Jul 27, 2018
Contact name Noah Hornick
Phone 503-494-0829
Organization name OHSU
Department Pediatric Hematology Oncology
Lab Peter Kurre
Street address 3181 S.W. Sam Jackson Park Rd.
City Portland
State/province OR
ZIP/Postal code 97239-3098
Country USA
Platforms (1)
GPL16384 [miRNA-3] Affymetrix Multispecies miRNA-3 Array
Samples (12)
GSM2283811 HL-60_exo-1
GSM2283812 HL-60_cel-1
GSM2283813 MOLM-14_exo-1
Reanalysis of GSM1328044
Reanalysis of GSM1328045
Reanalysis of GSM1328048
Reanalysis of GSM1328049
BioProject PRJNA339297

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE85769_RAW.tar 29.9 Mb (http)(custom) TAR (of CEL)
GSE85769_complete_normalized_data.txt.gz 1.4 Mb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table

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