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Series GSE85714 Query DataSets for GSE85714
Status Public on Jul 20, 2017
Title Molecular Analysis of Sink Limited Soybeans
Organism Glycine max
Experiment type Expression profiling by high throughput sequencing
Summary Soybean plants that do not produce a sink, such as depodded or male sterile plants, exhibit physiological and morphological changes during the reproductive stages, including increased levels of nitrogen and starch in the leaves and a delayed senescence. To identify transcriptional changes that occur in leaves of sink-limited plants, we used RNAseq to compare gene expression levels in trifoliate leaves from depodded and ms6 male sterile plants and control plants. In sink-limited tissues, we observed a deferral of the expression of senescence-associated genes and a continued high expression of genes associated with the maturity phase of leaf development. We identified GO-terms associated with growth and development and storage protein in sink limited tissues. We also identified that the bHLH. ARFs, and SBP transcription factors were expressed in sink limited tissues while the senescing control leaves expressed WRKY and NAC transcription factors. We identified genes that were not expressed during normal leaf development but highly expressed in sink-limited plants, including the D4 “non-yellowing” gene. These changes highlighted several metabolic pathways that were involved in distinct modes of resource parttioning in the “stay green” leaves.
Overall design Timecourse gene expression analysis of sink-limited soybean leaves. Gene expression was profiled from R2 growth stage (flowering) until the onset of leaf seenscence, and contrasted between mechanically and genetically sink-limited soybeans.
Contributor(s) Brown AV, Hudson KA
Citation(s) 28722115
Submission date Aug 16, 2016
Last update date May 15, 2019
Contact name Karen A Hudson
Organization name USDA-ARS
Department Crop Production and Pest Control
Street address 915 West State Street
City West Lafayette
State/province IN
ZIP/Postal code 47907
Country USA
Platforms (1)
GPL19246 Illumina HiSeq 2500 (Glycine max)
Samples (42)
GSM2282102 SterileAP1.1
GSM2282103 SterileAP1.2
GSM2282104 SterileAP1.3
BioProject PRJNA339152
SRA SRP082228

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Supplementary file Size Download File type/resource
GSE85714_Normalized_Depodding_data.csv.gz 3.3 Mb (ftp)(http) CSV
GSE85714_Normalized_Sterile_data.csv.gz 1.6 Mb (ftp)(http) CSV
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