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Series GSE8561 Query DataSets for GSE8561
Status Public on Dec 27, 2007
Title Mutant2
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary Cells in glucose-limited Saccharomyces cerevisiae cultures differentiate into quiescent (Q) and non-quiescent (NQ) fractions prior to entering stationary phase. To identify genes involved in this differentiation, Q and NQ cells from 101 deletion-mutant strains were tested for viability and reproductive capacity. Twenty-one mutants were identified, including 7 that affected reproductive capacity of both cell types. Thirteen affected only Q or NQ cells, indicating significant differentiation of these cell types. doa4 strains, lacking ubiquitin hydrolase, affected viability and reproductive capacity in both cell types. More than 1300 mRNAs differentiating Q and NQ cell fractions were identified by microarray analysis. Gene-ontology analysis of Q-cell mRNAs showed significant increases in protein-encoding mRNAs involved in membrane maintenance, oxidative stress response, and signal transduction. NQ-cell mRNAs encode proteins involved in Ty-element transposition and DNA recombination, consistent with apoptosis in these cells. Consistent with preparation for rapid response to environmental stimuli, approximately 2000 protease-labile mRNAs were identified in Q cells. The differentiation of these cell types and the ability of genes to selectively affect the survival of Q or NQ cells in yeast are relevant to chronological aging, cell-cycle, genome-evolution, and stem-cell research and provides insight into complex responses that even simple organisms have to starvation.
Keywords: Cell tyep comparison
 
Overall design Yeast deletion mutants (BY4742 background) were grown to stationary-phase (7 days) and then separted into Q and NQ cells using Percoll density gradients. Microarrays were carried out on these different cell fractions.
 
Contributor(s) Aragon AD, Werner-Washburne M
Citation(s) 18199684
Submission date Jul 23, 2007
Last update date Mar 19, 2012
Contact name Sushmita Roy
E-mail(s) sroy@cs.unm.edu
Phone 5052779340
Organization name Biology, University of New Mexico
Street address
City Albuquerque
State/province NM
ZIP/Postal code 87106
Country USA
 
Platforms (1)
GPL5627 Yeast 70mer oligoset by4742
Samples (190)
GSM212305 BY4742 Quiescent-Replicate ACS1
GSM212306 BY4742 Quiescent-Replicate ADH2
GSM212307 BY4742 Quiescent-Replicate ADY2
This SubSeries is part of SuperSeries:
GSE8624 Quiescent and non-quiescent deletion-mutant strains
Relations
BioProject PRJNA105381

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8561_RAW.tar 1.5 Gb (http)(custom) TAR (of TIFF)
Raw data included within Sample table
Raw data provided as supplementary file
Processed data included within Sample table

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