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Series GSE85527 Query DataSets for GSE85527
Status Public on Dec 06, 2016
Title Partially exhausted CD8+ T cells are associated with clinically beneficial response to Teplizumab in new onset type I diabetes (single-cell RNA-seq of sorted CD8+ T-cells)
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Biologic agents active in other autoimmune settings have had variable effectiveness in newly diagnosed type 1 diabetes (T1D) where treatment across therapeutic targets is accompanied by transient stabilization of C-peptide levels in some patients, followed by progression at the same rate as in control groups. Why disparate treatments lead to similar clinical courses is currently unknown. Here, we use integrated systems biology and flow cytometry approaches to elucidate immunologic mechanisms associated with C-peptide stabilization in T1D subjects treated with the anti-CD3 monoclonal antibody, teplizumab. This work is part of the Immune Tolerance Network AbATE study (Autoimmunity-Blocking Antibody for Tolerance in Recently Diagnosed Type 1 Diabetes); data are also available through the ITN TrialShare portal: https://www.itntrialshare.org/project/Studies/ITN027AIDB/Study%20Data/begin.view?.
 
Overall design We performed single-cell RNA-seq on 219 captured and sorted CD8+ T-cells from 3 responders at visit month 6 (N = 87, 75, 57); we sorted cells with and without EOMES-associated inhibitory receptors (TIGIT, KLRG1)); prior to RNA extraction, cells were stimulated with anti-CD3 and anti-CD28.
 
Contributor(s) Long SA, Thorpe J, DeBerg HA, Gersuk V, Eddy JA, Harris KM, Ehlers MR, Herold KC, Nepom GT, Linsley PS
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Submission date Aug 12, 2016
Last update date Mar 20, 2019
Contact name Scott Presnell
E-mail SPresnell@benaroyaresearch.org
Organization name Benaroya Research Institute
Street address 1201 Ninth Avenue
City Seattle
State/province WA
ZIP/Postal code 98101
Country USA
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (219)
GSM2274685 S32732_106306_hOKT3_R_6mo_CD8_TIGITpos_KLRG1pos_stim_untr_C25
GSM2274686 S32734_106306_hOKT3_R_6mo_CD8_TIGITpos_KLRG1pos_stim_untr_C08
GSM2274687 S32735_106306_hOKT3_R_6mo_CD8_TIGITpos_KLRG1pos_stim_untr_C20
This SubSeries is part of SuperSeries:
GSE85573 Partially exhausted CD8+ T cells are associated with clinically beneficial response to Teplizumab in new onset type I diabetes
Relations
BioProject PRJNA338798
SRA SRP081573

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE85527_AbATE_single_cell_profile_raw_counts.txt.gz 1.8 Mb (ftp)(http) TXT
GSE85527_metrics_AbATE_single_cell_libs.txt.gz 42.1 Kb (ftp)(http) TXT
Raw data are available in SRA
Processed data is available on Series record

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