|
| Status |
Public on May 02, 2017 |
| Title |
Regions of very low H3K27me3 partition the Drosophila genome into topological domains [HiC-seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
|
| Summary |
We have investigated boundaries of topologically associated domains (TADs) in the Drosophila genome and find that they can be identified as domains of very low H3K27me3. The genome-wide H3K27me3 profile partitions into two states; very low H3K27me3 identifies Depleted (D) domains that contain housekeeping genes and their regulators such as the histone acetyltransferase-containing NSL complex, whereas domains containing mid-to-high levels of H3K27me3 (Enriched or E domains) are associated with regulated genes, irrespective of whether they are active or inactive. The D domains correlate with the boundaries of TADs and are enriched in a subset of architectural proteins, particularly Chromator, BEAF-32, and Z4. However, rather than being clustered at the borders of these domains, these proteins bind throughout the H3K27me3-depleted regions and are much more strongly associated with the TSSs of the housekeeping genes than with the H3K27me3 domain boundaries. We suggest that the D domain chromatin state, characterised by very low H3K27me3 and established by housekeeping gene regulators, acts to separate topological domains thereby setting up the domain architecture of the genome.
|
| |
|
| Overall design |
Analysis of 3D chromatin organization using Hi-C with DpnII in Drosophila Kc167 cells.
|
| |
|
| Contributor(s) |
Magbanua JP, Fischer B, White R |
| Citation(s) |
28282436 |
| |
| Submission date |
Aug 11, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Rob White |
| E-mail(s) |
rw108@cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Physiology, Development and Neuroscience
|
| Street address |
Downing Street
|
| City |
Cambridge |
| ZIP/Postal code |
CB23DY |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL15334 |
Illumina HiSeq 1000 (Drosophila melanogaster) |
|
| Samples (2) |
|
| This SubSeries is part of SuperSeries: |
| GSE85504 |
Regions of very low H3K27me3 partition the Drosophila genome into topological domains |
|
| Relations |
| BioProject |
PRJNA338723 |
| SRA |
SRP081603 |
