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| Status |
Public on Feb 01, 2017 |
| Title |
Comparison of gene expression response to neutron and x-ray irradiation using mouse blood |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Background: In the event of an improvised nuclear device detonation, the prompt radiation exposure would consist of γ rays plus a neutron component that would contribute to the total dose. As neutrons cause more complex and difficult to repair damage to cells that would result in more severe health burden to affected individuals, it is paramount to be able to estimate the contribution of neutrons to an estimated dose, to provide information for those making treatment decisions.
Results: Mice exposed to either 0.25 or 1 Gy of neutron or 1 or 4 Gy x-ray radiation were sacrificed at 1 or 7 days after exposure. Whole genome microarray analysis identified 7,285 and 5,045 differentially expressed genes in the blood of mice exposed to neutron or x-ray radiation, respectively. Neutron exposure resulted in mostly downregulated genes, whereas x-rays showed both down- and up-regulated genes. A total of 34 differentially expressed genes were regulated in response to all ≥1 Gy exposures at both times. Of these, 25 genes were consistently downregulated at days 1 and 7, whereas 9 genes, including the transcription factor E2f2, showed bi-directional regulation; being downregulated at day 1, while upregulated at day 7. Gene ontology analysis revealed that genes involved in nucleic acid metabolism processes were persistently downregulated in neutron irradiated mice, whereas genes involved in lipid metabolism were upregulated in x-ray irradiated animals. Most biological processes significantly enriched at both timepoints were consistently represented by either under- or over-expressed genes. In contrast, cell cycle processes were significant among down-regulated genes at day 1, but among up-regulated genes at day 7 after exposure to either neutron or x-rays. Cell cycle genes downregulated at day 1 were mostly distinct from the cell cycle genes upregulated at day 7. However, five cell cycle genes, Fzr1, Ube2c, Ccna2, Nusap1, and Cdc25b, were both downregulated at day 1 and upregulated at day 7.
Conclusions: We describe, for the first time, the gene expression profile of mouse blood cells following exposure to neutrons. We have found that neutron radiation results in both distinct and common gene expression patterns compared with x-ray radiation.
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| Overall design |
Radiation induced gene expression in mouse blood was measured at 1, 3, and 7 days after 0.25 Gy and 1 Gy neutron exposure or after 1 Gy and 4 Gy of x-rays. Six independent experiments were performed at each dose and time point.
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| Contributor(s) |
Broustas CG, Amundson SA |
| Citation(s) |
28049433 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| U19 AI067773 |
CENTER FOR HIGH-THROUGHPUT MINIMALLY-INVASIVE DOSIMETRY: Center for High-Throughput Minimally-Invasive Radiation Biodosimetry: Center for High-Throughput Minimally-Invasive Radiation Biodosimetry: Center for High-Throughput Minimally-Invasive Radiation Biodosimetry: Center for High-Throughput Minimally-Invasive Radiation Biodosimetry: Center for High-Throughput Minimally-Invasive Radiation Biodosimetry: Center for High-Throughput Minimally-Invasive Radiation Biodosimetry: Center for High-Throughput Minimally-Invasive Radiation Biodosimetry |
Trustees of Columbia University in the City of New York |
DAVID JONATHAN BRENNER |
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| Submission date |
Aug 08, 2016 |
| Last update date |
Jan 19, 2018 |
| Contact name |
Sally Amundson |
| E-mail(s) |
saa2108@cumc.columbia.edu
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| Organization name |
Columbia University
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| Department |
Center for Radiological Research
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| Street address |
630 W. 168th St
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
| GPL10333 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version) |
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| Samples (72)
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| Relations |
| BioProject |
PRJNA338172 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE85323_RAW.tar |
652.5 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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