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| Status |
Public on Dec 10, 2016 |
| Title |
CTCF modulates Estrogen Receptor function through specific chromatin and nuclear matrix interactions [ChIP-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In our work we used high-throughput sequencing methods to get insight in the role of CTCF in ER-mediated gene regulation in luminal breast cancer cells. After assessing genome-wide binding of CTCF, ER, FOXA1 and Lamin B in MCF7 cells treated with estrogen for different time points, we could correlate the interaction to the chromatin with estrogen-induced de novo transcription (from GRO-seq data) and loop formation (from ChIA-PET, Fullwood et al., 2009). We observed that CTCF binding correlates with ER-mediated transcription and its depletion can affect ER-ER loop formation and subsequently gene expression.
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| Overall design |
Examination of CTCF, ER, FOXA1 and Lamin B chromatin binding in MCF7 cells upon estrogen stimulation for 0h, 45min or 3h.
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| |
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| Contributor(s) |
Fiorito E, Sharma Y, Gilfillan S |
| Citation(s) |
27638884 |
| |
| Submission date |
Aug 02, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Elisa Fiorito |
| E-mail(s) |
elisa.fiorito@ncmm.uio.no
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| Organization name |
University of Oslo (UiO)
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| Department |
Norwegian Centre for Molecular Medicine (NCMM)
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| Lab |
Breast Cancer group
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| Street address |
Forskningsparken - Gaustadalleen 21
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| City |
Oslo |
| ZIP/Postal code |
0349 |
| Country |
Norway |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE85108 |
CTCF modulates Estrogen Receptor function through specific chromatin and nuclear matrix interactions |
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| Relations |
| BioProject |
PRJNA336191 |
| SRA |
SRP080814 |
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