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Series GSE85106 Query DataSets for GSE85106
Status Public on Dec 10, 2016
Title CTCF modulates Estrogen Receptor function through specific chromatin and nuclear matrix interactions [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary In our work we used high-throughput sequencing methods to get insight in the role of CTCF in ER-mediated gene regulation in luminal breast cancer cells. After assessing genome-wide binding of CTCF, ER, FOXA1 and Lamin B in MCF7 cells treated with estrogen for different time points, we could correlate the interaction to the chromatin with estrogen-induced de novo transcription (from GRO-seq data) and loop formation (from ChIA-PET, Fullwood et al., 2009). We observed that CTCF binding correlates with ER-mediated transcription and its depletion can affect ER-ER loop formation and subsequently gene expression.
Overall design Examination of CTCF, ER, FOXA1 and Lamin B chromatin binding in MCF7 cells upon estrogen stimulation for 0h, 45min or 3h.
Contributor(s) Fiorito E, Sharma Y, Gilfillan S
Citation(s) 27638884
Submission date Aug 02, 2016
Last update date May 15, 2019
Contact name Elisa Fiorito
Organization name University of Oslo (UiO)
Department Norwegian Centre for Molecular Medicine (NCMM)
Lab Breast Cancer group
Street address Forskningsparken - Gaustadalleen 21
City Oslo
ZIP/Postal code 0349
Country Norway
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (15)
GSM2257816 CTCF_E0h_ChIPseq
GSM2257817 CTCF_E45m_ChIPseq
GSM2257818 CTCF_E3h_ChIPseq
This SubSeries is part of SuperSeries:
GSE85108 CTCF modulates Estrogen Receptor function through specific chromatin and nuclear matrix interactions
BioProject PRJNA336191
SRA SRP080814

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Supplementary file Size Download File type/resource
GSE85106_RAW.tar 4.5 Mb (http)(custom) TAR (of BED)
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Raw data are available in SRA
Processed data provided as supplementary file

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