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Series GSE85002 Query DataSets for GSE85002
Status Public on Dec 01, 2017
Title Inhibition of TGFβRI as a therapy for GATA4 deficient lung cancers [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary GATA4 is frequently epigenetically silenced in lung cancers. However, the impact of GATA4 inactivation on tumorigenesis and related therapeutic strategy remain to be determined. Through the genome-wide screening of tumor suppressing transcription factors, we demonstrate that GATA4 functions as an essential tumor suppressor in lung cancer in vitro and in vitro. Interestingly, ectopic GATA4 expression resulted in cellular senescence. Mechanistically, GATA4 up-regulates multiple miRNAs (miRNA-32, miRNA-301b, miR-320a, and miR-590) targeting TGFB2 mRNA and causes ensuing downregulation of WNT7B level to induce senescence. TGFBRI inhibitor synergizes with MEK1/2 inhibitor to promote lung cancer regression in Kras G12D/GATA4-/- mouse models. Decreased GATA4 level in clinical specimens negatively correlates with WNT7B or TGFB2 level and is significantly associated with poor prognosis.
 
Overall design Investigate GATA4 binding sequences by ChIP-seq
 
Contributor(s) Chen L, Gao L, Huang H
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Submission date Jul 29, 2016
Last update date May 15, 2019
Contact name huanwei huang
E-mail(s) hhw2436@gmail.com
Organization name NIBS
Street address 7 Science Park Rd., ZGC life science park
City Beijing
ZIP/Postal code 102206
Country China
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (2)
GSM2255735 GATA4
GSM2255736 IgG
This SubSeries is part of SuperSeries:
GSE85003 Inhibition of TGFβRI as a therapy for GATA4 deficient lung cancers
Relations
BioProject PRJNA335854
SRA SRP080351

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE85002_RAW.tar 370.0 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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