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Series GSE84982 Query DataSets for GSE84982
Status Public on Jul 30, 2016
Title Different responses of Caco-2 and MCF-7 cells to silver nanoparticles are based on highly similar mechanisms of action
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The mode of action of silver nanoparticles (AgNPs) is suggested to be exerted through both Ag+ and AgNP dependent mechanisms. Ingestion is one of the major NP exposure routes, and potential effects are often studied using Caco-2 cells, a well-established model for the gut epithelium. MCF-7 cells are epithelial breast cancer cells with extensive well-characterized toxicogenomics profiles. In the present study we aimed to gain a deeper understanding of the cellular molecular responses in Caco-2 and MCF-7 cells after AgNP exposure in order to evaluate whether epithelial cells derived from different tissues demonstrated similar responses. These insights could possibly reduce the size of cell panels for NP hazard identification screening purposes. AgNPs of 20, 30, 60, and 110 nm, and AgNO3 were exposed for 6h and 24h. AgNPs were shown to be taken up and dissolve intracellularly. Compared with MCF-7 cells, Caco-2 cells showed a higher sensitivity to AgNPs, slower gene expression kinetics, and absence of NP size-dependent responses. However, on a molecular level, no significant differences were observed between the two cell types. Transcriptomic analysis showed that Ag(NP) exposure caused (oxidative) stress responses, possibly leading to cell death in both cell lines. There was no indication for effects specifically induced by AgNPs. Responses to AgNPs appeared to be induced by silver ions released from the AgNPs. In conclusion, differences in mRNA responses to AgNPs between Caco-2 and MCF-7 cells were mainly related to timing and magnitude, but not to a different underlying mechanism.
Overall design In total 73 samples are analyzed (24 different samples all n=3; except for 1 sample with n=4).
Twelve of the 24 different samples are extracted from Caco-2 cells, 6 samples at t=6h and 6 at t=24h
The other 12 different of the 24 samples are extracted from MCF-7 cells, 6 samples at t=6h and 6 at t=24h
For each cell type and each timepoint one of the 6 samples was a negative control sample
Contributor(s) van der Zande M, Undas AK, Kramer E, Monopoli MP, Peters RJ, Garry D, Antunes Fernandes EC, Hendriksen PJ, Marvin HJ, Peijnenburg AA, Bouwmeester H
Citation(s) 27597447
Submission date Jul 29, 2016
Last update date Aug 13, 2018
Contact name Meike van der Zande
Organization name Wageningen University & Research
Street address Akkermaalsbos 2
City Wageningen
ZIP/Postal code 6708AK
Country Netherlands
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (73)
GSM2255360 Caco2_ctrl_6h_rep1
GSM2255361 Caco2_ctrl_6h_rep2
GSM2255362 Caco2_ctrl_6h_rep3
BioProject PRJNA335824

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE84982_RAW.tar 26.2 Mb (http)(custom) TAR
GSE84982_non-normalized.txt.gz 20.3 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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