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Series GSE8471 Query DataSets for GSE8471
Status Public on Feb 08, 2008
Title MCF7 HRG stimulation
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Heregulin beta-1 (HRG) is an extracellular ligand that activates mitogen-activated protein kinase (MAPK) and phosphatidylinositol-3-OH kinase (PI3K)/Akt signaling pathways through ErbB receptors. MAPK and Akt have been shown to phosphorylate the estrogen receptor (ER) at Ser-118 and Ser-167, respectively, thereby mimicking the effects of estrogenic activity such as estrogen responsive element (ERE)-dependent transcription. In the current study, integrative analysis was performed using two tiling array platforms, comprising histone H3 lysine 9 (H3K9) acetylation and RNA mapping, together with array comparative genomic hybridization (CGH) analysis in an effort to identify HRG-regulated genes in ER-positive MCF-7 breast cancer cells. Through application of various threshold settings, 333 (326 up-regulated and 7 down-regulated) HRG-regulated genes were detected. Prediction of upstream transcription factors (TFs) and pathway analysis indicated that 21% of HRG-induced gene regulation may be controlled by the MAPK cascade, while only 0.6% of the gene expression is controlled by ERE. A comparison with previously reported estrogen (E2)-regulated gene expression data revealed that only 12 common genes were identified between the 333 HRG-regulated (3.6%) and 239 E2-regulated (5.0%) gene groups. However, with respect to enriched upstream TFs, 4 common TFs were identified in the 14 HRG-regulated (28.6%) and 13 E2-regulated (30.8%) gene groups. These results indicated that while E2 and HRG may induce common TFs, the regulatory mechanisms that govern HRG- and E2-induced gene expression differ.
Keywords: growth hormone response
 
Overall design MCF-7 human breast cancer cells were incubated 2hour after administration of 10nM of the growth hormones (heregulin (HRG)). Control was set as growth hormone non-treated cells.
 
Citation(s) 18350142
Submission date Jul 13, 2007
Last update date Jan 08, 2019
Contact name Mariko Okada
E-mail marikoh@rcai.riken.jp
Organization name RIKEN RCAI
Lab Laboratory for Cellular Systems Modeling
Street address W518, 1-7-22, Suehiro-cho, Tsurumi-ku
City Yokohama
ZIP/Postal code 230-0045
Country Japan
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (2)
GSM210180 MCF7 Control
GSM210181 MCF7 HRG 2h
Relations
BioProject PRJNA101553

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Supplementary file Size Download File type/resource
GSE8471_RAW.tar 16.5 Mb (http)(custom) TAR (of CEL, EXP)
Raw data provided as supplementary file
Processed data included within Sample table

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