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| Status |
Public on Dec 01, 2017 |
| Title |
ChIP-seq analysis of Rad21 binding during genome activation |
| Organism |
Danio rerio |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The formation of a transcriptional competent zygote is a not fully understood process potentially involving changes in chromatin structure. Cohesin and CTCF are important nuclear architectural proteins that contribute to chromatin structure and gene regulation. Here we analyze the genome-wide location of Rad21 binding during zebrafish embryogenesis and show increased Rad21 binding over developmental time (2.5 hpf, 4.5 hpf , 10 hpf).
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| |
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| Overall design |
ChIP-seq to generate genome wide binding data for Rad21 in three stages of zebrafish development, two biological replicates for each stage with input controls.
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| Contributor(s) |
Meier M, Horsfield JA |
| Citation(s) |
29158440 |
| |
| Submission date |
Jul 20, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Michael Meier |
| Organization name |
University of Otago
|
| Department |
Pathology
|
| Lab |
Chromosome Structure and Development
|
| Street address |
1st Floor Hercus Building, Dunedin School of Medicine, Great King Street
|
| City |
Dunedin |
| ZIP/Postal code |
9012 |
| Country |
New Zealand |
| |
|
| Platforms (2) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
| GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
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| Samples (14)
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| This SubSeries is part of SuperSeries: |
| GSE84602 |
Cohesin facilitates zygotic genome activation in Zebrafish |
|
| Relations |
| BioProject |
PRJNA330617 |
| SRA |
SRP078973 |
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