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| Status |
Public on Jul 12, 2017 |
| Title |
Reconstructing blood stem cell regulatory network models from single-cell molecular profiles |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The aim of this study was to validate networks for haematopoietic stem and progenitor cell networks, using ChIP-Seq data to assess and confirm the genomic binding sites of important haematopoietic transcription factors in a haematopoietic cell line.
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| Overall design |
HoxB8-FL cells were fixed with either 1% or 0.4% formaldehyde for 10 mins. Chromatin was isolated, sonicated for 7 mins (30 sec on, 30 sec off), and specific antibodies were used to pull down the transcription factors of interest after a pre-clearing step. Chromatin was washed, de-crosslinked, amplified, size selected by gel purification and sequenced.
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| Contributor(s) |
Nestorowa S, Hamey F, Kinston SJ, Kent D, Wilson NK, Gottgens B, Hannah R |
| Citation(s) |
28584094 |
| |
| Submission date |
Jul 12, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Bertie Gottgens |
| E-mail(s) |
bg200@cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Street address |
Jeffrey Cheah Biomedical Centre
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0AW |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA328869 |
| SRA |
SRP078378 |
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