|
| Status |
Public on Jan 31, 2008 |
| Title |
Expression survey of Rpp1 soybean line PI200492 resistant to P. pachyrhizi |
| Organism |
Glycine max |
| Experiment type |
Expression profiling by array
|
| Summary |
Affymetrix soybean genome arrays were used to identify genes differentially expressed in the immune resistance response at 6, 12, 24, and 48 hours after inoculation with Phakopsora pachyrhizi isolates TW72-1 or HW94-1
soybean + HW94-1 = resistant; soybean + TW72-1 = susceptible
Keywords: time course
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| Overall design |
For each of 4 time points (6, 12, 24 and 48 hours post inoculation) a soybean PI200492 plant was inoculated with 2 treatments: HW94-1, or TW72-1. A mock inoculation was performed and sampled at time 0. There were three biological replicates of the entire experiment, for a total of 27 samples. During data analysis two samples failed the quality control metrics and were removed from the analysis, leaving 25 samples in the final data set.
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| |
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| Contributor(s) |
Schneider KT, Choi JJ, Alkharouf NW, Lum NL, Munroe DJ, Matthews BF, Frederick RD |
| Citation(s) |
17906332 |
| Submission date |
Jul 10, 2007 |
| Last update date |
Nov 22, 2016 |
| Contact name |
katherine schneider |
| E-mail(s) |
katherine.schneider@ars.usda.gov
|
| Phone |
301-619-5256
|
| Fax |
301-619-2880
|
| Organization name |
USDA
|
| Department |
FDWSRU
|
| Lab |
Frederick
|
| Street address |
1301 Ditto Ave
|
| City |
Ft. Detrick |
| State/province |
MD |
| ZIP/Postal code |
21702 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL4592 |
[Soybean] Affymetrix Soybean Genome Array |
|
| Samples (27)
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| Relations |
| BioProject |
PRJNA101495 |
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