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| Status |
Public on Oct 13, 2017 |
| Title |
Mechanisms of HERV-K (HML-2) transcription during human mammary epithelial cell transformation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Elevated transcript expression of the endogenous retrovirus family HERV-K (HML-2) is seen in the majority of breast cancers, although the identity of the individual loci contributing to this expression as well as their mechanism of activation is unclear. Using high-throughput next-generation sequencing techniques optimized for the capture of HML-2 expression, we produced a complete profile of the HML-2 transcriptome before and after human mammary epithelial cell transformation.
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| |
|
| Overall design |
Using Illumina MiSeq sequencing, we profiled the HERV-K (HML-2) mRNA expression in four human mammary epithelial cell lines of varying tumorigenicity.
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| |
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| Contributor(s) |
Montesion M, Coffin JM |
| Citation(s) |
29046454, 30126415 |
| Submission date |
Jul 11, 2016 |
| Last update date |
May 17, 2019 |
| Contact name |
John M Coffin |
| E-mail(s) |
john.coffin@tufts.edu
|
| Organization name |
Tufts University
|
| Department |
Molecular Biology & Microbiology
|
| Street address |
136 Harrison Ave.
|
| City |
Boston |
| ZIP/Postal code |
02111 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA328504 |
| SRA |
SRP078272 |
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