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Series GSE84213 Query DataSets for GSE84213
Status Public on Jun 30, 2017
Title BET proteins bind the lytic origins of replication [MutuI ChIP-seq]
Organisms Homo sapiens; Human gammaherpesvirus 4
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Lytic infection by the Epstein-Barr virus (EBV) poses numerous health risks, such as infectious mononucleosis and lymphoproliferative disorder. We demonstrate that JQ1 and other BET inhibitors block two different steps in the sequential cascade of the EBV life cycle: expression of the immediate-early gene BZLF1 and lytic genome replication. This represents a novel mode of action for antiviral drugs that may increase efficacy and decrease emergence of resistance. The ChIP-seq data below show that the BET proteins bind to both EBV lytic origins of replication.
 
Overall design ChIP-seq and input from untreated and JQ1 treated MutuI cells
 
Contributor(s) Keck KM, Moquin SA, Miranda JL
Citation(s) 28588024
Submission date Jul 10, 2016
Last update date May 15, 2019
Contact name JJ Miranda
E-mail(s) jmiranda@barnard.edu
Organization name Barnard College, Columbia University
Street address 3009 Broadway
City New York
ZIP/Postal code 10027
Country USA
 
Platforms (3)
GPL22131 Illumina HiSeq 2500 (Homo sapiens; Human herpesvirus 4)
GPL22132 Illumina HiSeq 4000 (Homo sapiens; Human herpesvirus 4)
GPL23362 Illumina HiSeq 4000 (Homo sapiens; Human gammaherpesvirus 4)
Samples (18)
GSM2229480 input_Mutu_Replicate1
GSM2229481 input_Mutu_Replicate2
GSM2229482 ChIP-seq_BRD4_Mutu_Replicate1
This SubSeries is part of SuperSeries:
GSE84214 BET inhibitors block the Epstein-Barr virus lytic cycle at two distinct steps
Relations
BioProject PRJNA328409
SRA SRP078255

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE84213_RAW.tar 19.0 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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