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| Status |
Public on Sep 26, 2018 |
| Title |
The NFkB subunit RELA is a master transcriptional regulator of the committed epithelial-mesenchymal transition in airway epithelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The epithelial-mesenchymal transition (EMT) is a multistep dedifferentiation program important in tissue repair. Here, we examined the role of the transcriptional regulator NFkB in EMT of human primary small airway epithelial cells (hSAECs). Surprisingly, transforming growth factor β (TGFβ) activated NFkB/RELA proto-oncogene, NFkB subunit (RELA) translocation within 1 day of stimulation, yet induction of its downstream gene regulatory network occurred only after 3 days. A time course of TGFβ-induced EMT transition was analyzed by RNA-Seq in the absence or presence of inducible shRNA-mediated silencing of RELA. In WT cells, TGFβ stimulation significantly affected the expression of 2,441 genes. Gene set enrichment analysis identified Wnt, cadherin, and NFkB signaling as the most prominent TGFβ-inducible pathways. By comparison, RELA controlled expression of 3,138 overlapping genes mapping to Wnt, cadherin, and chemokine signaling pathways. Conducting upstream regulator analysis, we found that RELA controls six clusters of upstream transcription factors, many of which overlapped with a transcription factor topology map of EMT developed earlier. RELA triggered expression of three key EMT pathways: (1) the Wnt/β-catenin morphogen pathway, (2) the JUN transcription factor, and (3) the Snail family transcriptional repressor 1 (SNAI1). RELA binding to target genes was confirmed by ChIP. Experiments independently validating Wnt dependence on RELA were performed by silencing RELA via genome editing and indicated that TGFβ-induced WNT5B expression and downstream activation of the Wnt target AXIN2 are RELA-dependent. We conclude that RELA is a master transcriptional regulator of EMT upstream of Wnt morphogen, JUN, SNAI1-ZEB1, and interleukin-6 autocrine loops.
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| Overall design |
RNA-seq transcriptome profiling of TGF-Beta stimulated RelA wildtype and knock-down cells
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| Contributor(s) |
Brasier AR, Widen SG |
| Citation(s) |
30166344 |
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| Submission date |
Jul 07, 2016 |
| Last update date |
May 22, 2019 |
| Contact name |
Allan Brasier |
| E-mail(s) |
abrasier@wisc.edu
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| Phone |
409-772-2824
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| Organization name |
University Texas Medical Branch
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| Department |
Medicine
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| Street address |
301 Univ. Blvd
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| City |
Galveston |
| State/province |
TX |
| ZIP/Postal code |
77555-1060 |
| Country |
USA |
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| Platforms (1) |
| GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA328132 |
| SRA |
SRP078053 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE84135_DESeq_rld.counts.tsv.gz |
3.3 Mb |
(ftp)(http) |
TSV |
| GSE84135_all.counts.tsv.gz |
708.1 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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