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| Status |
Public on Jul 04, 2018 |
| Title |
Gene expression in microglia of WT and Trem2(-/-) mice upon treatment with cuprizone, Covi-Ox, or both |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Eight groups of mice of two genetic backgrounds (WT or Trem2(-/-)) were fed one of the four diets: 1) regular chow; 2) chow supplemented with Covi-ox T-70; 3) chow supplemented with cuprizone; 4) chow supplemented with both Covi-Ox and cuprizone
After this, the differences in the brain of experimental animals were characterized with microscopy and microarray analysis of gene expression.
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| Overall design |
Mice were fed the diet ad libitum. Mice fed cuprizone and tocopherols were placed on the Covi-ox T70 with additional tocopherols diet for 2 weeks prior to feeding with Covi-ox T70 and cuprizone together for either 4 additional weeks or 7 weeks followed by 1 week of normal chow diet. Mice fed cuprizone only were maintained on normal chow for 2 weeks and then changed to cuprizone containing feed for 4 weeks or 7 weeks followed by 1 week of normal chow diet. After the mice were sacrificed, microglia were sorted, and gene expression was analyzed.
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| Contributor(s) |
Predeus A, Ulland T, Wang Y, Arbuzov I, Song W, Lampropoulou V, Bambouskova M, Towfic F, Gilfillan S, Loginicheva E, Edelson B, Zeskind B, Colonna M, Artyomov M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jul 06, 2016 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Alexander Predeus |
| E-mail(s) |
predeus@gmail.com
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| Phone |
+447472882494
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| Organization name |
Wellcome Sanger Institute
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| Street address |
Wellcome Trust Genome Campus
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| City |
Hinxton |
| ZIP/Postal code |
CB10 1RQ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA328041 |
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