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| Status |
Public on Feb 15, 2017 |
| Title |
Gene expression of liver sinusoidal endothelial cells from an 8-week-old WT and ob/ob mouse |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Obesity may promote myeloid cell accumulation in liver. Intravital liver tissue imaging revealed that the number of myeloid cells attached to sinusoidal wall significantly increased in ob/ob mice. To assess the pathophysiological roll of liver sinusoidal endothelial cells (LSEC) in obese livers, we performed microarray analysis in LSEC isolated from an 8-week-old wild-type and ob/ob mouse. Pathway analysis in microarray data shows genes related to cell adhesion, chemokine signaling and leukocyte transendothelial migration are upregulated in LSEC from ob/ob.
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| Overall design |
Liver sinusoidal endothelial cells were isolated by collagenage digestion of livers from an 8-week-old male wild-type and ob/ob mouse. Cells were cultured overnight in DMEM containing 10% FBS.
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| Contributor(s) |
Miyachi Y, Yamaguchi S, Tsuchiya K, Ogawa Y |
| Citation(s) |
28297678 |
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| Submission date |
Jul 05, 2016 |
| Last update date |
Jan 19, 2018 |
| Contact name |
Yasutaka Miyachi |
| E-mail(s) |
ymiyachi.mem@tmd.ac.jp
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| Organization name |
Tokyo Medical and Dental University
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| Department |
Molecular Endocrinology and Metabolism
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| Street address |
1-5-45 Yushima
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| City |
Bunko-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
1138510 |
| Country |
Japan |
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| Platforms (1) |
| GPL10333 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA327826 |
