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Series GSE83965 Query DataSets for GSE83965
Status Public on Aug 04, 2016
Title Sox2 suppresses gastric tumorigenesis in mice [CHIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Purpose: Sox2 expression marks gastric stem and progenitor cells, raising important questions regarding the genes regulated by Sox2 and the role of Sox2 itself during stomach homeostasis and disease. The goal of this study is to determine the function of and the genes regulated by Sox2 in the stomach.
Methods: Sox2 ChIP-enriched DNA and input DNA was isolated from gastric glands of adult antrum from Sox2 KO and Sox2 WT mice. DNA was purified and genomic libraries were prepared as described (Sulahian et al., 2014), using four micrograms of goat anti-SOX2 (AF2018, R&D). Libraries were sequenced (50 bp, single-end reads) on an Illumina Hi-Seq 2000 instrument.
Results: Sox2 is dispensiable for gastric stem cell self-renewal and epithelial homeostasis, however modulates the expression of wnt, intestinal and cancer related genes
 
Overall design Examination of Sox2 targets in the stomachs of Sox2 WT and Sox2 KO mice.
 
Contributor(s) Anselmo A, Hochedlinger K
Citation(s) 27498859
Submission date Jul 01, 2016
Last update date May 15, 2019
Contact name Abby Sarkar
Organization name Harvard University
Department Cancer Center
Lab Konrad Hochedlinger
Street address 185 Cambridge Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (3)
GSM2224576 Wild type Stomach [ChIP-seq]
GSM2224577 Sox2 KO Stomach [ChIP-seq]
GSM2224578 Wild type Stomach [input]
This SubSeries is part of SuperSeries:
GSE83966 Sox2 suppresses gastric tumorigenesis in mice
Relations
BioProject PRJNA327572
SRA SRP077733

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE83965_RAW.tar 120.0 Kb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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