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| Status |
Public on Sep 20, 2016 |
| Title |
Genome-wide HP1a occupancy measured by DamID-seq |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In order to map levels of genome-wide HP1a occupancy we applied DamID (van Steensel & Henikoff, Nat Biotech, 2000; PMID: 10748524) in combination with next-generation sequencing of methylated GATC fragments. Mapping by next-generation sequencing makes it possible to examine heterochromatic regions that were not covered by earlier datasets which were generated using microarrays.
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| Overall design |
Wild-type Kc167 cells were transiently transfected in duplicates with Dam-HP1a or Dam only and collected by two days after transfection for quantification of genome-wide methylation levels via next-geneartion sequencing.
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| Contributor(s) |
Brueckner L, van Arensbergen J, Akhtar W, Pagie L, van Steensel B |
| Citation(s) |
27777628 |
| |
| Submission date |
Jun 24, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Bas van Steensel |
| E-mail(s) |
b.v.steensel@nki.nl
|
| Phone |
+ 31 20 512 2040
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| Fax |
+31 20 669 1383
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| URL |
http://www.nki.nl/nkidep/vansteensel
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| Organization name |
Netherlands Cancer Institute
|
| Department |
division of Molecular Biology
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| Lab |
van Steensel group
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| Street address |
Plesmanlaan 121
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| City |
Amsterdam |
| ZIP/Postal code |
1066 CX |
| Country |
Netherlands |
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| Platforms (1) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA326778 |
| SRA |
SRP077069 |
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