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Status |
Public on Jun 26, 2017 |
Title |
Transcript profiling of Saccharomyces cerevisiae cells overexpressing YOX1 before and after 2 and 4 hours of hydroxyurea (HU) treatment (200 mM) |
Organism |
Saccharomyces cerevisiae |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
mRNA processing bodies (P-bodies) are cytoplasmic granules that form in eukaryotic cells in response to numerous stresses to serve as sites of degradation and storage of mRNAs. Functional P-bodies are critical for the DNA replication stress response in yeast, yet the repertoire of P-body targets and the mechanisms by which P-bodies promote replication stress resistance are unknown. In this study, we identify the complete complement of mRNA targets of P-bodies during replication stress induced by hydroxyurea treatment. The key P-body protein Lsm1 controls the abundance of HHT1, ACF4, ARL3, TMA16, RRS1 and YOX1 mRNAs to prevent their toxic accumulation during replication stress. Accumulation of YOX1 mRNA causes aberrant down-regulation of a network of genes critical for DNA replication stress resistance. Among these genes we identify ALD6, whose de-repression is critical to prevent accumulation of acetaldehyde, a strongly toxic molecule during DNA replication stress.
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Overall design |
WT containing pBY011 (control) or pBY011-YOX1 vectors were cultured in synthetic medium containing raffinose as unique carbon source. Cultures were then treated with hydroxyurea (HU) at a final concentration of 200 mM and galactose was added at the same time to induce YOX1 overexpression. Samples were collected before and after 2 and 4h of galactose induction and hydroxyurea treatment. Experiment was repeated twice.
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Contributor(s) |
Loll-Krippleber R, Brown GW |
Citation(s) |
28916784 |
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Submission date |
Jun 16, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Raphael Loll-Krippleber |
E-mail(s) |
raphael.loll.krippleber@gmail.com
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Organization name |
University of Toronto
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Department |
Biochemistry
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Street address |
160 College street
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City |
Toronto |
State/province |
Ontario |
ZIP/Postal code |
M5S3E1 |
Country |
Canada |
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Platforms (1) |
GPL17342 |
Illumina HiSeq 2500 (Saccharomyces cerevisiae) |
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Samples (12)
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GSM2203528 |
WT cells + pBY011 control vector - untreated condition - replicate 1 |
GSM2203529 |
WT cells + pBY011 control vector - untreated condition - replicate 2 |
GSM2203530 |
WT cells + pBY011-YOX1 vector - untreated condition - replicate 1 |
GSM2203531 |
WT cells + pBY011-YOX1 vector - untreated condition - replicate 2 |
GSM2203532 |
WT cells + pBY011 control vector - 2h galactose induction and HU treatment - replicate 1 |
GSM2203533 |
WT cells + pBY011 control vector - 2h galactose induction and HU treatment - replicate 2 |
GSM2203534 |
WT cells + pBY011-YOX1 vector - 2h galactose induction and HU treatment - replicate 1 |
GSM2203535 |
WT cells + pBY011-YOX1 vector - 2h galactose induction and HU treatment - replicate 2 |
GSM2203536 |
WT cells + pBY011 control vector - 4h galactose induction and HU treatment - replicate 1 |
GSM2203537 |
WT cells + pBY011 control vector - 4h galactose induction and HU treatment - replicate 2 |
GSM2203538 |
WT cells + pBY011-YOX1 vector - 4h galactose induction and HU treatment - replicate 1 |
GSM2203539 |
WT cells + pBY011-YOX1 vector - 4h galactose induction and HU treatment - replicate 2 |
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This SubSeries is part of SuperSeries: |
GSE83515 |
P-bodies regulate transcriptional rewiring to promote DNA replication stress resistance |
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Relations |
BioProject |
PRJNA326241 |
SRA |
SRP076798 |