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| Status |
Public on Jun 26, 2017 |
| Title |
Transcript profiling of WT and lsm1∆ Saccharomyces cells before and after 1, 2 and 4 hours of hydroxyurea treatment (200 mM) |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
mRNA processing bodies (P-bodies) are cytoplasmic granules that form in eukaryotic cells in response to numerous stresses to serve as sites of degradation and storage of mRNAs. Functional P-bodies are critical for the DNA replication stress response in yeast, yet the repertoire of P-body targets and the mechanisms by which P-bodies promote replication stress resistance are unknown. In this study, we identify the complete complement of mRNA targets of P-bodies during replication stress induced by hydroxyurea treatment. The key P-body protein Lsm1 controls the abundance of HHT1, ACF4, ARL3, TMA16, RRS1 and YOX1 mRNAs to prevent their toxic accumulation during replication stress. Accumulation of YOX1 mRNA causes aberrant down-regulation of a network of genes critical for DNA replication stress resistance. Among these genes we identify ALD6, whose de-repression is critical to prevent accumulation of acetaldehyde, a strongly toxic molecule during DNA replication stress.
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| Overall design |
WT and lsm1∆ cells were cultured in YPD. Sample were taken before and after 1, 2 and 4 hours of treatment with hydroxurea at a final concentration of 200 mM. Experiment was repeated twice.
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| Contributor(s) |
Loll-Krippleber R, Brown GW |
| Citation(s) |
28916784 |
| |
| Submission date |
Jun 16, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Raphael Loll-Krippleber |
| E-mail(s) |
raphael.loll.krippleber@gmail.com
|
| Organization name |
University of Toronto
|
| Department |
Biochemistry
|
| Street address |
160 College street
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5S3E1 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL17342 |
Illumina HiSeq 2500 (Saccharomyces cerevisiae) |
|
| Samples (16)
|
| GSM2203512 |
WT cells - untreated condition - replicate 1 |
| GSM2203513 |
WT cells - untreated condition - replicate 2 |
| GSM2203514 |
lsm1∆ cells - untreated condition - replicate 1 |
| GSM2203515 |
lsm1∆ cells - untreated condition - replicate 2 |
| GSM2203516 |
WT cells - 1h HU 200 mM - replicate 1 |
| GSM2203517 |
WT cells - 1h HU 200 mM - replicate 2 |
| GSM2203518 |
lsm1∆ cells - 1h HU 200 mM - replicate 1 |
| GSM2203519 |
lsm1∆ cells - 1h HU 200 mM - replicate 2 |
| GSM2203520 |
WT cells - 2h HU 200 mM - replicate 1 |
| GSM2203521 |
WT cells - 2h HU 200 mM - replicate 2 |
| GSM2203522 |
lsm1∆ cells - 2h HU 200 mM - replicate 1 |
| GSM2203523 |
lsm1∆ cells - 2h HU 200 mM - replicate 2 |
| GSM2203524 |
WT cells - 4h HU 200 mM - replicate 1 |
| GSM2203525 |
WT cells - 4h HU 200 mM - replicate 2 |
| GSM2203526 |
lsm1∆ cells - 4h HU 200 mM - replicate 1 |
| GSM2203527 |
lsm1∆ cells - 4h HU 200 mM - replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE83515 |
P-bodies regulate transcriptional rewiring to promote DNA replication stress resistance |
|
| Relations |
| BioProject |
PRJNA326236 |
| SRA |
SRP076792 |
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