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Series GSE8275 Query DataSets for GSE8275
Status Public on Sep 01, 2007
Title Non-additive and imprinted gene expression in hybrid maize endosperm_13DAP
Organism Zea mays
Experiment type Expression profiling by array
Summary The nuclear content of the plant endosperm is the result of the contribution two maternal genomes and a single paternal genome. This 2:1 dosage relationship provides a unique system for studying the additivity of gene expression levels in reciprocal hybrids. A combination of microarray profiling and allele-specific expression analysis was performed using RNA isolated from endosperm tissues of maize inbred lines B73 and Mo17 and their reciprocal hybrids at two developmental stages, 13 and 19 days after pollination. By assessing the relative levels of expression in the reciprocal hybrids it was possible to determine the prevalence of additive and non-additive expression patterns. While the majority of differentially expressed genes displayed additive expression patterns in the endosperm, approximately 10% of the genes displayed non-additive expression patterns including maternal-like, paternal-like, dominant high-parent, dominant low-parent and expression patterns outside the range of the inbreds. The frequency of hybrid expression patterns outside of the parental range in maize endosperm tissue is much higher than that observed for vegetative tissues. For a set of 90 genes allele-specific expression assays were employed to monitor allelic bias and regulatory variation. Eight of these genes exhibited evidence for maternally or paternally biased expression at multiple stages of endosperm development and are potential examples of differential imprinting. Collectively, our data indicate that parental effects on gene expression are much stronger in endosperm than in vegetative tissues, and that endosperm imprinting may be far more common than previously estimated.
Keywords: genotype comparison
Overall design Affymetrix microarrays were used to perform expression profiling on 13 day after pollination endosperm tissue of four different genotypes; B73; Mo17, B73xMo17 and Mo17xB73. There are three biological replicates for each of the tissues. Each biological sample represents a pool containing 5 endosperms each, from 6 different ears.
Contributor(s) Springer NM, Stupar RM, Hermanson PJ
Citation(s) 17766400
Submission date Jun 25, 2007
Last update date Mar 19, 2012
Contact name Nathan M Springer
Phone 6126246241
Fax 6126251738
Organization name University of Minnesota
Department Plant Biology
Street address 1445 Gortner Ave
City Saint Paul
State/province MN
ZIP/Postal code 55108
Country USA
Platforms (1)
GPL4032 [Maize] Affymetrix Maize Genome Array
Samples (12)
GSM205365 Mo17 13 DAP endosperm tissue biological replicate 1
GSM205366 B73 13 DAP endosperm tissue biological replicate 1
GSM205367 Mo17xB73 13 DAP endosperm tissue biological replicate 1
This SubSeries is part of SuperSeries:
GSE8308 Non-additive and imprinted gene expression in hybrid maize endosperm_13DAP and 19DAP
BioProject PRJNA105427

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8275_RAW.tar 112.8 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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