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Series GSE8248 Query DataSets for GSE8248
Status Public on Aug 17, 2007
Title Identification of hypoxia-inducible genes in Arabidopsis mesophyll cells
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary The goal of this experiment was to investigate the early transcript changes (6h) induced by hypoxia treatment in mesophyll protoplasts. A single pair (control & hypoxia) of GeneChipsĀ® was used to confirm that hypoxia treatment altered the expression of an overlapping set of genes controlled by KIN10 (At3g01090) in Arabidopsis mesophyll protoplasts.
Keywords: KIN10, KIN11, darkness, hypoxia, starvation, stress, sugar signalling, Arabidopsis, SnRK1
 
Overall design For the hypoxic treatment protoplasts were submerged in mannitol buffer and the hypoxia condition was confirmed by the activation of the well-established early marker genes ADH (alcohol dehydrogenase, At1g77120) and PDC1 (pyruvate decarboxylase-1, At4g33070) based on RT-PCR and GeneChipĀ® analyses. Results show a clear overlap in genes induced under hypoxic conditions and those induced by KIN10 expression in protoplasts. The overlap of the gene expression profiles was confirmed by triplicated real-time quantitative RT-PCR analyses of selected marker genes.
 
Contributor(s) Baena-Gonzalez E, Rolland F, Thevelein JM, Sheen J
Citation(s) 17671505
Submission date Jun 22, 2007
Last update date Aug 28, 2018
Contact name Elena Baena-Gonzalez
E-mail(s) baena@molbio.mgh.harvard.edu
Organization name Massachusetts General Hospital and Harvard Medical School
Department Molecular Biology (MGH) and Department of Genetics (HMS)
Lab Jen Sheen
Street address Simches Research Building, 185 Cambridge Street, CPZN7250
City Boston
State/province MA
ZIP/Postal code 02114-2790
Country USA
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (2)
GSM204026 protoplast_control_rep1
GSM204069 protoplast_hypoxia_rep1
Relations
BioProject PRJNA101191

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8248_RAW.tar 6.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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