|
| Status |
Public on Oct 04, 2016 |
| Title |
BRD4 localization to lineage-specific enhancers is associated with a distinct transcription factor repertoire [ChIP-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We examined context specific function of BRD4 in promoting lineage specific gene expression and show that BRD4 is essential for osteoblast differentiation.
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| |
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| Overall design |
We performed ChIP-sequencing from hFOB cells (undifferentiated and differentiated for 5 days into osteoblastic lineage) . We generated BRD4 and RNA POLII ChIP-Seq data from undifferentiated and differentiated cells; H3K27ac, H3K27me3 and H2Bub1 ChIP-Seq data from undifferentiated and differentiated cells with DMSO or JQ1 treatment. Consistently, Input DNA from all the three conditions was included as well. C/EBPb, TEAD1, FOSL2, JUND and STAT3 ChIP-Seq was performed in differentiated hFOBs .
We performed ChIP-sequencing from L3.6 cells. We generated BRD4 ChIP-Seq data.
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| |
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| Contributor(s) |
Johnsen SA, Najafova Z |
| Citation(s) |
27651452 |
| |
| Submission date |
Jun 06, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Steven A. Johnsen |
| Organization name |
Robert Bosch Center for Tumor Diseases
|
| Lab |
Molecular Cancer Epigenetics
|
| Street address |
Auerbachstraße 112
|
| City |
Stuttgart |
| State/province |
BW |
| ZIP/Postal code |
70376 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (59)
|
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| This SubSeries is part of SuperSeries: |
| GSE82297 |
BRD4 localization to lineage-specific enhancers is associated with a distinct transcription factor repertoire |
|
| Relations |
| BioProject |
PRJNA324577 |
| SRA |
SRP076178 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE82295_Merged_DIF_BRD4.bigwig |
180.5 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_DIF_CEBPb.bigwig |
202.0 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_DIF_FOSL2.bigwig |
65.5 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_DIF_H2Bub1.bigwig |
125.5 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_DIF_H3K27ac.bigwig |
115.5 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_DIF_H3K27me3.bigwig |
135.9 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_DIF_INPUT.bigwig |
247.2 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_DIF_JQ1_H2Bub1.bigwig |
115.0 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_DIF_JQ1_H3K27ac.bigwig |
126.2 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_DIF_JQ1_H3K27me3.bigwig |
134.0 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_DIF_JQ1_INPUT.bigwig |
182.7 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_DIF_JUND.bigwig |
67.0 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_DIF_RNAPII.bigwig |
168.3 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_DIF_STAT3.bigwig |
66.8 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_DIF_TEAD1.bigwig |
67.4 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_L3.6_BRD4.bigwig |
103.5 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_L3.6_INPUT.bigwig |
211.9 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_UND_BRD4.bigwig |
188.4 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_UND_H2Bub1.bigwig |
119.5 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_UND_H3K27ac.bigwig |
98.0 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_UND_H3K27me3.bigwig |
134.5 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_UND_INPUT.bigwig |
247.9 Mb |
(ftp)(http) |
BIGWIG |
| GSE82295_Merged_UND_RNAPII.bigwig |
153.5 Mb |
(ftp)(http) |
BIGWIG |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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