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Series GSE82295 Query DataSets for GSE82295
Status Public on Oct 04, 2016
Title BRD4 localization to lineage-specific enhancers is associated with a distinct transcription factor repertoire [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We examined context specific function of BRD4 in promoting lineage specific gene expression and show that BRD4 is essential for osteoblast differentiation.
 
Overall design We performed ChIP-sequencing from hFOB cells (undifferentiated and differentiated for 5 days into osteoblastic lineage) . We generated BRD4 and RNA POLII ChIP-Seq data from undifferentiated and differentiated cells; H3K27ac, H3K27me3 and H2Bub1 ChIP-Seq data from undifferentiated and differentiated cells with DMSO or JQ1 treatment. Consistently, Input DNA from all the three conditions was included as well. C/EBPb, TEAD1, FOSL2, JUND and STAT3 ChIP-Seq was performed in differentiated hFOBs .
We performed ChIP-sequencing from L3.6 cells. We generated BRD4 ChIP-Seq data.
 
Contributor(s) Johnsen SA, Najafova Z
Citation(s) 27651452
Submission date Jun 06, 2016
Last update date May 15, 2019
Contact name Steven A. Johnsen
Organization name Mayo Clinic
Department Division of Gastroenterology and Hepatology
Lab Gene Regulatory Mechanisms and Molecular Epigenetics Lab
Street address 200 First St SW
City Rochester
State/province MN
ZIP/Postal code 55905
Country USA
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (59)
GSM2188457 DIF_INPUT_A
GSM2188458 DIF_INPUT_B
GSM2188459 DIF_INPUT_C
This SubSeries is part of SuperSeries:
GSE82297 BRD4 localization to lineage-specific enhancers is associated with a distinct transcription factor repertoire
Relations
BioProject PRJNA324577
SRA SRP076178

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE82295_Merged_DIF_BRD4.bigwig 180.5 Mb (ftp)(http) BIGWIG
GSE82295_Merged_DIF_CEBPb.bigwig 202.0 Mb (ftp)(http) BIGWIG
GSE82295_Merged_DIF_FOSL2.bigwig 65.5 Mb (ftp)(http) BIGWIG
GSE82295_Merged_DIF_H2Bub1.bigwig 125.5 Mb (ftp)(http) BIGWIG
GSE82295_Merged_DIF_H3K27ac.bigwig 115.5 Mb (ftp)(http) BIGWIG
GSE82295_Merged_DIF_H3K27me3.bigwig 135.9 Mb (ftp)(http) BIGWIG
GSE82295_Merged_DIF_INPUT.bigwig 247.2 Mb (ftp)(http) BIGWIG
GSE82295_Merged_DIF_JQ1_H2Bub1.bigwig 115.0 Mb (ftp)(http) BIGWIG
GSE82295_Merged_DIF_JQ1_H3K27ac.bigwig 126.2 Mb (ftp)(http) BIGWIG
GSE82295_Merged_DIF_JQ1_H3K27me3.bigwig 134.0 Mb (ftp)(http) BIGWIG
GSE82295_Merged_DIF_JQ1_INPUT.bigwig 182.7 Mb (ftp)(http) BIGWIG
GSE82295_Merged_DIF_JUND.bigwig 67.0 Mb (ftp)(http) BIGWIG
GSE82295_Merged_DIF_RNAPII.bigwig 168.3 Mb (ftp)(http) BIGWIG
GSE82295_Merged_DIF_STAT3.bigwig 66.8 Mb (ftp)(http) BIGWIG
GSE82295_Merged_DIF_TEAD1.bigwig 67.4 Mb (ftp)(http) BIGWIG
GSE82295_Merged_L3.6_BRD4.bigwig 103.5 Mb (ftp)(http) BIGWIG
GSE82295_Merged_L3.6_INPUT.bigwig 211.9 Mb (ftp)(http) BIGWIG
GSE82295_Merged_UND_BRD4.bigwig 188.4 Mb (ftp)(http) BIGWIG
GSE82295_Merged_UND_H2Bub1.bigwig 119.5 Mb (ftp)(http) BIGWIG
GSE82295_Merged_UND_H3K27ac.bigwig 98.0 Mb (ftp)(http) BIGWIG
GSE82295_Merged_UND_H3K27me3.bigwig 134.5 Mb (ftp)(http) BIGWIG
GSE82295_Merged_UND_INPUT.bigwig 247.9 Mb (ftp)(http) BIGWIG
GSE82295_Merged_UND_RNAPII.bigwig 153.5 Mb (ftp)(http) BIGWIG
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Raw data are available in SRA
Processed data are available on Series record

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