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| Status |
Public on Jan 01, 2017 |
| Title |
Differences in transcriptomic profiles between glioblastoma-initiating cells (GICs) and non-GICs upon radiation treatment |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
GICs are considered a major determinant in glioblastoma treatment failure, as they exhibit a marked resistance to radiotherapy. However, the exact mechanisms that confer this radioresistance to GICs are still not fully understood. We have performed a microarray analysis after radiation treatment of GICs and non-GICs cultures with the aim of identifying genes, pathways and transcription factors differentially expressed between these two cellular compartments.
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| Overall design |
GICs and non-GICs cultures derived from a glioblastoma patient named as PG35s and PG35, respectively, were irradiated with fractionated radiotherapy at a dose of 2Gy/day up to 8Gy. After recovery, GICs once irradiated were exposed to a second cycle of radiation. Then, total RNA was extracted from both, controls and irradiated conditions. Three independent experiments were performed using different batches for each sample.
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| Contributor(s) |
Tortosa A, Stanzani E, Martinez J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 01, 2016 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Miguel Angel Pujana |
| E-mail(s) |
mapujana@iconcologia.net
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| Phone |
0034932607463
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| Organization name |
Institut Catala d'Oncologia IDIBELL
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| Lab |
ProCURE
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| Street address |
Gran Via 199
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| City |
Barcelona |
| ZIP/Postal code |
08908 |
| Country |
Spain |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA324121 |
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