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Series GSE8166 Query DataSets for GSE8166
Status Public on Dec 01, 2007
Title Transcriptome Profile of the Vascular Endothelial Cell Response to Candida albicans
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We perform microarray analysis of HUVECs upon stimulation with virulent wildtype C. albicans strain SC5314 or its efg1/efg1 cph1/cph1 hyphal-deficient derivative strain CAN34 to compare the gene expression profiles elicited from HUVECs in response to these strains. In addition, these responses are compared to that of TNF-alpha induced responses to determine which responses are Candida-specific.
Keywords: comparison of host response to different Candida albicans morphologies
 
Overall design HUVECs are co-cultured for 3 or 8 hours in M199 medium alone or with CAN34, SC5314, or TNF-alpha. Total RNA is isolated, cRNA is synthesized and labeled, and labeled cRNA is hybridized onto Affymetrix chips. Each Candida and TNF sample is compared to its corresponding medium alone sample to determine fold changes in expression at each time point.
 
Contributor(s) Barker KS, Park H, Phan QT, Xu L, Homayouni R, Liu TT, Rogers PD, Filler SG
Citation(s) 18500935
Submission date Jun 18, 2007
Last update date Jan 08, 2019
Contact name Katherine S Barker
E-mail ksbarker@uthsc.edu
Organization name University of Tennessee Health Science Center
Department Clinical Pharmacy
Lab David Rogers laboratory
Street address 881 Madison Avenue, Room 305
City Memphis
State/province TN
ZIP/Postal code 38163
Country USA
 
Platforms (2)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (20)
GSM202141 HUVEC vs CAN34 3hr exposure 3rd expt
GSM202144 HUVEC vs CAN34 8hr exposure 3rd expt
GSM202176 HUVEC vs M199 3hr exposure 3rd expt
Relations
BioProject PRJNA101061

Download family Format
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Supplementary file Size Download File type/resource
GSE8166_RAW.tar 150.0 Mb (http)(custom) TAR (of CEL, CHP, EXP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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