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Series GSE81651 Query DataSets for GSE81651
Status Public on Jun 15, 2016
Title ERRalpha mediates metabolic adaptations that drive lapatinib resistance in breast cancer (ERRalpha binding profiles in breast cancer cells by ChIP-seq)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Ablation of ERRalpha significantly delays ERBB2-induced mammary tumorigenesis and ERRalpha regulates genes of the ERBB2 amplicon. To further investigate the relationship between ERRalpha activity and RTK signaling, we mapped ERRalpha binding sites in SKBr3 cells upon EGF treatment or heregulin treatment. Inhibition of ERBB2 signaling using the RTK inhibitor lapatinib impacts on ERRalpha stability, while cells resistant to lapatinib treatment exhibit restored ERRalpha expression. We therefore mapped ERRalpha binding sites in parental (sensitive) cells (pSKBr3) as well as in lapatinib-resistant cells (LRSKBr3).
 
Overall design ChIP-Seq analysis of ERRalpha binding profile in SKBr3 or BT-474 breast cancer cells.
 
Contributor(s) Giguere V
Citation(s) 27402251
Submission date May 19, 2016
Last update date May 15, 2019
Contact name Vincent Giguère
E-mail(s) vincent.giguere@mcgill.ca
Phone 514-398-5899
Organization name McGill University
Department Goodman Cancer Centre
Street address 1160 Pine Ave West, McIntyre Bldg Room 710
City Montréal
State/province QC
ZIP/Postal code H3A 1A3
Country Canada
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (19)
GSM2160172 SKBr3_nt_ERRaChIP_Replicate1
GSM2160173 SKBr3_nt_ERRaChIP_Replicate2
GSM2160174 SKBr3_nt_Input
Relations
BioProject PRJNA322202
SRA SRP075427

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE81651_BT474_egf_G26_peaks.bed.gz 394.0 Kb (ftp)(http) BED
GSE81651_BT474_hrg_G27_peaks.bed.gz 603.8 Kb (ftp)(http) BED
GSE81651_BT474_nt_G25_peaks.bed.gz 475.1 Kb (ftp)(http) BED
GSE81651_LRSKBr3_G31_G34_G36_ERRChIP_peaks.bed.gz 580.0 Kb (ftp)(http) BED
GSE81651_SKBr3_EGF_G6_G23_G2_ERRChIP_peaks.bed.gz 476.9 Kb (ftp)(http) BED
GSE81651_SKBr3_hrg_G8_G24_G3_ERRChIP_peaks.bed.gz 542.0 Kb (ftp)(http) BED
GSE81651_SKBr3_nt_G4_G22_G1_ERRChIP_peaks.bed.gz 470.2 Kb (ftp)(http) BED
GSE81651_pSKBr3_G29_G32_G35_ERRChIP_peaks.bed.gz 329.8 Kb (ftp)(http) BED
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Raw data are available in SRA
Processed data are available on Series record

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