 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 20, 2016 |
| Title |
The effect of antibiotic mediated gut microbiome perturbation on ileal gene expression in NOD mice. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The early life microbiome plays important roles in host immunological and metabolic development. Because type 1 diabetes (T1D) incidence has been increasing substantially in recent decades, we hypothesized that early-life antibiotic use alters gut microbiota that predisposes to disease. Using NOD mice that are genetically susceptible to T1D, we examined the effects of exposure to either continuous low-dose antibiotics or pulsed therapeutic antibiotics (PAT) early in life, mimicking childhood exposures. We found that in mice receiving PAT, T1D incidence was significantly higher, microbial community composition and structure differed compared with controls. In pre-diabetic male PAT mice, the intestinal lamina propria had lower Th17 and T reg proportions and intestinal SAA expression than in controls, suggesting key roles in transducing the altered microbiota signals. PAT affected microbial lipid metabolism and host cholesterol biosynthetic gene expression. These findings show that early-life antibiotic treatments alter the gut microbiota and its metabolic capacities, intestinal gene expression, and T-cell populations, accelerating T1D onset in NOD mice.
|
| |
|
| Overall design |
Microarray experiment was performed on RNA extracted from the terminal ileum of control; STAT; and PAT mice. (Female n=3/group; Male n=5/group except STAT n=3).
|
| |
|
| Contributor(s) |
Livanos AE, Greiner TU, Vangay P, Pathmasiri W, Stewart D, McRitchie S, Li H, Chung J, Sohn J, Kim S, Gao Z, Barber C, Kim J, Ng S, Rogers AB, Sumner S, Zhang X, Cadwell K, Knights D, Alekseyenko A, Backhed F, Blaser MJ |
| Citation(s) |
27782139 |
| |
| Submission date |
May 19, 2016 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Thomas W Battaglia |
| Organization name |
NYU School of Medicine
|
| Department |
Medicine
|
| Lab |
Blaser Lab
|
| Street address |
423 E 23rd Street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10010 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (22)
|
|
| Relations |
| BioProject |
PRJNA322196 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE81648_RAW.tar |
80.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...